On the isolation of polychlorinated dibenzo-p-dioxins and furans from serum samples using immunoaffinity chromatography prior to high-resolution gas chromatography-mass spectrometry

Janice K. Huwe, Weilin L. Shelver, Larry Stanker, Donald George Jr Patterson, Wayman E. Turner

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Immunoaffinity chromatography (IAC) for the purification of polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs) from biological samples was explored as a means to simplify the cleanup procedure and thereby decrease the time and cost of dioxin analysis. A monoclonal antibody (DD3) was used to produce IAC columns and to isolate the PCDD/Fs from serum. Native and 13C-labeled PCDD/Fs were spiked at the ppq to ppt range into serum. Quantitation of the PCDD/Fs was performed by a standard dioxin analytical method, i.e. high-resolution gas chromatography-mass spectrometry (GC-MS), which was easily compatible with IAC. Five of the most toxic PCDD/Fs consistently showed acceptable recoveries (>25%) and were reliably quantitated. The congeners specifically recovered by this method represent almost 80% of the toxic equivalency of dioxins and furans present in the serum samples. Dioxin-like polychlorinated biphenyls (PCBs) were not recognized by this antibody column. Compared to conventional dioxin cleanup methods, IAC decreased solvent usage by 1.5 l/sample and took only 2 h to process a sample for analysis.

Original languageEnglish (US)
Pages (from-to)285-293
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume757
Issue number2
DOIs
StatePublished - Jun 15 2001

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Furans
Chromatography
Dioxins
Gas chromatography
Mass spectrometry
Poisons
Column chromatography
Polychlorinated Biphenyls
Purification
dibenzo(1,4)dioxin
Monoclonal Antibodies
Recovery
Antibodies
Costs

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

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title = "On the isolation of polychlorinated dibenzo-p-dioxins and furans from serum samples using immunoaffinity chromatography prior to high-resolution gas chromatography-mass spectrometry",
abstract = "Immunoaffinity chromatography (IAC) for the purification of polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs) from biological samples was explored as a means to simplify the cleanup procedure and thereby decrease the time and cost of dioxin analysis. A monoclonal antibody (DD3) was used to produce IAC columns and to isolate the PCDD/Fs from serum. Native and 13C-labeled PCDD/Fs were spiked at the ppq to ppt range into serum. Quantitation of the PCDD/Fs was performed by a standard dioxin analytical method, i.e. high-resolution gas chromatography-mass spectrometry (GC-MS), which was easily compatible with IAC. Five of the most toxic PCDD/Fs consistently showed acceptable recoveries (>25{\%}) and were reliably quantitated. The congeners specifically recovered by this method represent almost 80{\%} of the toxic equivalency of dioxins and furans present in the serum samples. Dioxin-like polychlorinated biphenyls (PCBs) were not recognized by this antibody column. Compared to conventional dioxin cleanup methods, IAC decreased solvent usage by 1.5 l/sample and took only 2 h to process a sample for analysis.",
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On the isolation of polychlorinated dibenzo-p-dioxins and furans from serum samples using immunoaffinity chromatography prior to high-resolution gas chromatography-mass spectrometry. / Huwe, Janice K.; Shelver, Weilin L.; Stanker, Larry; Patterson, Donald George Jr; Turner, Wayman E.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 757, No. 2, 15.06.2001, p. 285-293.

Research output: Contribution to journalArticle

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