TY - JOUR
T1 - On the origin of mRNA encoding the truncated dopamine D3-type receptor D(3nf) and detection of D(3nf)-like immunoreactivity in human brain
AU - Liu, K.
AU - Bergson, C.
AU - Levenson, R.
AU - Schmauss, C.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1994
Y1 - 1994
N2 - A truncated dopamine D3-receptor-like mRNA, named D(3nf), predicts a protein that differs from the D3-receptor only in the carboxyl terminus. However, such a protein has lost the predicted membrane topology typically found for G protein-coupled receptors. Results presented here show that D(3nf) mRNA arises from the D3-encoded primary transcript via alternative splicing. This splicing, however, appears to involve cleavage of an unusual 3' splice site. Therefore, we tested the possibility that D(3nf) mRNA results from a splicing error. If this were the case, D(3nf) mRNA would be expected to be present in the cytoplasm only at very low amounts, and it would not be expected to be translated into protein. However, the relative abundance of cytoplasmic D3/D(3nf) mRNA in human cortical tissues was found to be similar. Furthermore, we raised polyclonal antisera against the predicted carboxyl-terminal peptide sequence of D(3nf) that reacts specifically with a protein expressed in stably D(3nf) mRNA-expressing COS 7 cells. The use of this antiserum also revealed the presence of a ~68 kDa D(3nf)-like immunoreactive protein in human brain, suggesting that the atypically processed D(3nf) mRNA is translated.
AB - A truncated dopamine D3-receptor-like mRNA, named D(3nf), predicts a protein that differs from the D3-receptor only in the carboxyl terminus. However, such a protein has lost the predicted membrane topology typically found for G protein-coupled receptors. Results presented here show that D(3nf) mRNA arises from the D3-encoded primary transcript via alternative splicing. This splicing, however, appears to involve cleavage of an unusual 3' splice site. Therefore, we tested the possibility that D(3nf) mRNA results from a splicing error. If this were the case, D(3nf) mRNA would be expected to be present in the cytoplasm only at very low amounts, and it would not be expected to be translated into protein. However, the relative abundance of cytoplasmic D3/D(3nf) mRNA in human cortical tissues was found to be similar. Furthermore, we raised polyclonal antisera against the predicted carboxyl-terminal peptide sequence of D(3nf) that reacts specifically with a protein expressed in stably D(3nf) mRNA-expressing COS 7 cells. The use of this antiserum also revealed the presence of a ~68 kDa D(3nf)-like immunoreactive protein in human brain, suggesting that the atypically processed D(3nf) mRNA is translated.
UR - http://www.scopus.com/inward/record.url?scp=0028113535&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028113535&partnerID=8YFLogxK
M3 - Article
C2 - 7961889
AN - SCOPUS:0028113535
VL - 269
SP - 29220
EP - 29226
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 46
ER -