A total of 1,025,415 bases of nucleotide sequence, including the entire human immunoglobulin λ gene locus has been determined. This is the largest contiguous human DNA sequence ever published. The sequence data revealed the organization of 36 potentially active V(λ) gene segments, 33 pseudogene segments, and several J(λ)-C(λ) gene segments. Among these 69 functional or nonfunctional V(λ) gene segments, 32 were newly discovered. These V(λ) gene segments are located within five gene-rich clusters and are divided into five clans based on sequence identity. Five potentially active nonimmunoglobulin genes were also detected within the λ gene locus, and two other genes were observed in the upstream region. Sequence organization suggests that large DNA duplications diversified the germ-line repertoire of the V(λ) gene segments.
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