Cleaning-in-place (CIP) is widely used on dairy farms to clean and sanitize milking system components after the milking event is completed. An increasing number of dairy farms is adopting a one-step CIP, which combines the alkaline wash and acid wash cycles together as one wash cycle. This one-step CIP saves time, energy, and chemical consumption. Electrolyzed oxidizing (EO) water is an emerging technology producing alkaline and acidic EO water by electrolyzing dilute sodium chloride solution. Previous studies have shown that blending the alkaline EO water with the acidic EO water at a certain ratio could be used as an alternative one-step CIP. Therefore, this study was to evaluate the deposit removal during the one-step CIP wash using the already optimized blended EO water solution on the stainless steel surface evaluation simulator. Stainless steel straight pipes were used as specimens and the remaining milk deposit mass on the inner surfaces of the specimens was evaluated. A two-term exponential decay kinetic model was developed for one-step blended EO water wash with a fast deposit removal rate in the beginning of the wash cycle along with a slow deposit removal rate throughout the entire one-step wash. The proposed models agreed the experimental data with acceptable root mean square errors. The relative light unit (RLU) reading from ATP bioluminescence was used as an indirect evaluation at each time sampling point during the one-step wash cycle. Scanning electron microscopy (SEM) was used to understand the residual deposit morphology and qualitatively evaluate the specimen inner surfaces cleanliness after the one-step wash. Results showed that specimens at the completion of the blended EO water one-step wash could be considered as clean as indicated from the RLU cutoff reading of stainless steel material and the developed mathematical model for the blended EO water one-step wash was acceptable for the simulator application.