Oomycete species associated with soybean seedlings in North America-part I: Identification and pathogenicity characterization

J. Alejandro Rojas, Janette L. Jacobs, Stephanie Napieralski, Behirda Karaj, Carl A. Bradley, Thomas Chase, Paul Esker, Loren J. Giesler, Doug J. Jardine, Dean K. Malvick, Samuel G. Markell, Berlin D. Nelson, Alison E. Robertson, John C. Rupe, Damon L. Smith, Laura E. Sweets, Albert U. Tenuta, Kiersten A. Wise, Martin I. Chilvers

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Oomycete pathogens are commonly associated with soybean root rot and have been estimated to reduce soybean yields in the United States by 1.5 million tons on an annual basis. Limited information exists regarding the frequency and diversity of oomycete species across the major soybean-producing regions in North America. A survey was conducted across 11 major soybean-producing states in the United States and the province of Ontario, Canada. In 2011, 2,378 oomycete cultures were isolated from soybean seedling roots on a semiselective medium (CMAPARPB) and were identified by sequencing of the internal transcribed spacer region of rDNA. Sequence results distinguished a total of 51 Pythium spp., three Phytophthora spp., three Phytopythium spp., and one Aphanomyces sp. in 2011, with Pythium sylvaticum (16%) and P. oopapillum (13%) being the most prevalent. In 2012, the survey was repeated, but, due to drought conditions across the sampling area, fewer total isolates (n = 1,038) were collected. Additionally, in 2012, a second semiselective medium (V8-RPBH) was included, which increased the Phytophthora spp. isolated from 0.7 to 7% of the total isolates. In 2012, 54 Pythium spp., seven Phytophthora spp., six Phytopythium spp., and one Pythiogeton sp. were recovered, with P. sylvaticum (14%) and P. heterothallicum (12%) being recovered most frequently. Pathogenicity and virulence were evaluated with representative isolates of each of the 84 species on soybean cv. Sloan. A seed-rot assay identified 13 and 11 pathogenic species, respectively, at 13 and 20C. A seedling-root assay conducted at 20C identified 43 species as pathogenic, having a significantly detrimental effect on the seedling roots as compared with the noninoculated control. A total of 15 species were pathogenic in both the seed and seedling assays. This study provides a comprehensive characterization of oomycete species present in soybean seedling roots in the major production areas in the United States and Ontario, Canada and provides a basis for disease management and breeding programs.

Original languageEnglish (US)
Pages (from-to)280-292
Number of pages13
JournalPHYTOPATHOLOGY
Volume107
Issue number3
DOIs
StatePublished - Mar 1 2017

Fingerprint

Oomycetes
pathogenicity
soybeans
seedlings
Pythium sylvaticum
Phytophthora
Pythium
Ontario
assays
Canada
Aphanomyces
root rot
seeds
internal transcribed spacers
disease control
virulence
drought
pathogens
breeding

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Rojas, J. A., Jacobs, J. L., Napieralski, S., Karaj, B., Bradley, C. A., Chase, T., ... Chilvers, M. I. (2017). Oomycete species associated with soybean seedlings in North America-part I: Identification and pathogenicity characterization. PHYTOPATHOLOGY, 107(3), 280-292. https://doi.org/10.1094/PHYTO-04-16-0177-R
Rojas, J. Alejandro ; Jacobs, Janette L. ; Napieralski, Stephanie ; Karaj, Behirda ; Bradley, Carl A. ; Chase, Thomas ; Esker, Paul ; Giesler, Loren J. ; Jardine, Doug J. ; Malvick, Dean K. ; Markell, Samuel G. ; Nelson, Berlin D. ; Robertson, Alison E. ; Rupe, John C. ; Smith, Damon L. ; Sweets, Laura E. ; Tenuta, Albert U. ; Wise, Kiersten A. ; Chilvers, Martin I. / Oomycete species associated with soybean seedlings in North America-part I : Identification and pathogenicity characterization. In: PHYTOPATHOLOGY. 2017 ; Vol. 107, No. 3. pp. 280-292.
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Rojas, JA, Jacobs, JL, Napieralski, S, Karaj, B, Bradley, CA, Chase, T, Esker, P, Giesler, LJ, Jardine, DJ, Malvick, DK, Markell, SG, Nelson, BD, Robertson, AE, Rupe, JC, Smith, DL, Sweets, LE, Tenuta, AU, Wise, KA & Chilvers, MI 2017, 'Oomycete species associated with soybean seedlings in North America-part I: Identification and pathogenicity characterization', PHYTOPATHOLOGY, vol. 107, no. 3, pp. 280-292. https://doi.org/10.1094/PHYTO-04-16-0177-R

Oomycete species associated with soybean seedlings in North America-part I : Identification and pathogenicity characterization. / Rojas, J. Alejandro; Jacobs, Janette L.; Napieralski, Stephanie; Karaj, Behirda; Bradley, Carl A.; Chase, Thomas; Esker, Paul; Giesler, Loren J.; Jardine, Doug J.; Malvick, Dean K.; Markell, Samuel G.; Nelson, Berlin D.; Robertson, Alison E.; Rupe, John C.; Smith, Damon L.; Sweets, Laura E.; Tenuta, Albert U.; Wise, Kiersten A.; Chilvers, Martin I.

In: PHYTOPATHOLOGY, Vol. 107, No. 3, 01.03.2017, p. 280-292.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Oomycete species associated with soybean seedlings in North America-part I

T2 - Identification and pathogenicity characterization

AU - Rojas, J. Alejandro

AU - Jacobs, Janette L.

AU - Napieralski, Stephanie

AU - Karaj, Behirda

AU - Bradley, Carl A.

AU - Chase, Thomas

AU - Esker, Paul

AU - Giesler, Loren J.

AU - Jardine, Doug J.

AU - Malvick, Dean K.

AU - Markell, Samuel G.

AU - Nelson, Berlin D.

AU - Robertson, Alison E.

AU - Rupe, John C.

AU - Smith, Damon L.

AU - Sweets, Laura E.

AU - Tenuta, Albert U.

AU - Wise, Kiersten A.

AU - Chilvers, Martin I.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Oomycete pathogens are commonly associated with soybean root rot and have been estimated to reduce soybean yields in the United States by 1.5 million tons on an annual basis. Limited information exists regarding the frequency and diversity of oomycete species across the major soybean-producing regions in North America. A survey was conducted across 11 major soybean-producing states in the United States and the province of Ontario, Canada. In 2011, 2,378 oomycete cultures were isolated from soybean seedling roots on a semiselective medium (CMAPARPB) and were identified by sequencing of the internal transcribed spacer region of rDNA. Sequence results distinguished a total of 51 Pythium spp., three Phytophthora spp., three Phytopythium spp., and one Aphanomyces sp. in 2011, with Pythium sylvaticum (16%) and P. oopapillum (13%) being the most prevalent. In 2012, the survey was repeated, but, due to drought conditions across the sampling area, fewer total isolates (n = 1,038) were collected. Additionally, in 2012, a second semiselective medium (V8-RPBH) was included, which increased the Phytophthora spp. isolated from 0.7 to 7% of the total isolates. In 2012, 54 Pythium spp., seven Phytophthora spp., six Phytopythium spp., and one Pythiogeton sp. were recovered, with P. sylvaticum (14%) and P. heterothallicum (12%) being recovered most frequently. Pathogenicity and virulence were evaluated with representative isolates of each of the 84 species on soybean cv. Sloan. A seed-rot assay identified 13 and 11 pathogenic species, respectively, at 13 and 20C. A seedling-root assay conducted at 20C identified 43 species as pathogenic, having a significantly detrimental effect on the seedling roots as compared with the noninoculated control. A total of 15 species were pathogenic in both the seed and seedling assays. This study provides a comprehensive characterization of oomycete species present in soybean seedling roots in the major production areas in the United States and Ontario, Canada and provides a basis for disease management and breeding programs.

AB - Oomycete pathogens are commonly associated with soybean root rot and have been estimated to reduce soybean yields in the United States by 1.5 million tons on an annual basis. Limited information exists regarding the frequency and diversity of oomycete species across the major soybean-producing regions in North America. A survey was conducted across 11 major soybean-producing states in the United States and the province of Ontario, Canada. In 2011, 2,378 oomycete cultures were isolated from soybean seedling roots on a semiselective medium (CMAPARPB) and were identified by sequencing of the internal transcribed spacer region of rDNA. Sequence results distinguished a total of 51 Pythium spp., three Phytophthora spp., three Phytopythium spp., and one Aphanomyces sp. in 2011, with Pythium sylvaticum (16%) and P. oopapillum (13%) being the most prevalent. In 2012, the survey was repeated, but, due to drought conditions across the sampling area, fewer total isolates (n = 1,038) were collected. Additionally, in 2012, a second semiselective medium (V8-RPBH) was included, which increased the Phytophthora spp. isolated from 0.7 to 7% of the total isolates. In 2012, 54 Pythium spp., seven Phytophthora spp., six Phytopythium spp., and one Pythiogeton sp. were recovered, with P. sylvaticum (14%) and P. heterothallicum (12%) being recovered most frequently. Pathogenicity and virulence were evaluated with representative isolates of each of the 84 species on soybean cv. Sloan. A seed-rot assay identified 13 and 11 pathogenic species, respectively, at 13 and 20C. A seedling-root assay conducted at 20C identified 43 species as pathogenic, having a significantly detrimental effect on the seedling roots as compared with the noninoculated control. A total of 15 species were pathogenic in both the seed and seedling assays. This study provides a comprehensive characterization of oomycete species present in soybean seedling roots in the major production areas in the United States and Ontario, Canada and provides a basis for disease management and breeding programs.

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