Opiate nerves mediate feline pyloric response to intraduodenal amino acids

J. C. Reynolds, Ann Ouyang, S. Cohen

Research output: Contribution to journalArticle

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Abstract

Intraluminal pressures and myoelectric activity were recorded from the feline antrum, pylorus, and duodenum in response to intraduodenal amino acid solutions. Mixed amino acids (0.02 mg/ml, 3.0 ml) increased the amplitude of pyloric contractions (59.7 ± 7.9 mmHg) and pyloric spike activity (73.7 ± 6.8% of slow waves with spike activity) compared with a saline control (P < 0.001). The selectivity of these responses was determined with specific amino acids. L-Tryptophan (10 or 40 mM) produced a response similar to the mixed amino acid response, while L-phenylalanine or L-glycine (10 or 40 mM) had no effect. Intra-arterial tetrodotoxin, intraluminal ethyl aminobenzoate, or intravenous naloxone (1.0 mg/kg) abolished the pyloric responses to amino acids (P < 0.02). Bilateral cervical vagotomy had no effect. Cholecystokinin octapeptide (CCK-OP) produced dose-dependent increases in the amplitude of pyloric contractions and in pyloric spike activity. The ED 50 dose of CCK-OP (1.0 μg/kg iv) gave an increase in pyloric pressure of 155.6 ± 49.9 mmHg and in spike activity of 77.7 ± 9.4%, similar to mixed amino acids or tryptophan. These effects of CCK-OP were not antagonized, however, by a dose of naloxone (1.0 mg/kg) that blocked the maximal pyloric response to leucine-enkephalin. We concluded 1) intraduodenal mixed amino acids or tryptophan increase phasic, spike-dependent pyloric contractions in the cat via nonvagal, naloxone-sensitive neural pathways, 2) phenylalanine, a structurally similar essential amino acid, had no effect on the feline gastroduodenal junction, and 3) the pyloric responses to exogenous CCK-OP are mediated by pathways distinct from the responses to tryptophan or mixed amino acids.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume11
Issue number3
StatePublished - Jan 1 1985

Fingerprint

Opiate Alkaloids
Felidae
Amino Acids
Sincalide
Tryptophan
Naloxone
Phenylalanine
Benzocaine
Leucine Enkephalin
Pressure
Neural Pathways
Essential Amino Acids
Vagotomy
Pylorus
Tetrodotoxin
Duodenum
Glycine
Cats

All Science Journal Classification (ASJC) codes

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

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title = "Opiate nerves mediate feline pyloric response to intraduodenal amino acids",
abstract = "Intraluminal pressures and myoelectric activity were recorded from the feline antrum, pylorus, and duodenum in response to intraduodenal amino acid solutions. Mixed amino acids (0.02 mg/ml, 3.0 ml) increased the amplitude of pyloric contractions (59.7 ± 7.9 mmHg) and pyloric spike activity (73.7 ± 6.8{\%} of slow waves with spike activity) compared with a saline control (P < 0.001). The selectivity of these responses was determined with specific amino acids. L-Tryptophan (10 or 40 mM) produced a response similar to the mixed amino acid response, while L-phenylalanine or L-glycine (10 or 40 mM) had no effect. Intra-arterial tetrodotoxin, intraluminal ethyl aminobenzoate, or intravenous naloxone (1.0 mg/kg) abolished the pyloric responses to amino acids (P < 0.02). Bilateral cervical vagotomy had no effect. Cholecystokinin octapeptide (CCK-OP) produced dose-dependent increases in the amplitude of pyloric contractions and in pyloric spike activity. The ED 50 dose of CCK-OP (1.0 μg/kg iv) gave an increase in pyloric pressure of 155.6 ± 49.9 mmHg and in spike activity of 77.7 ± 9.4{\%}, similar to mixed amino acids or tryptophan. These effects of CCK-OP were not antagonized, however, by a dose of naloxone (1.0 mg/kg) that blocked the maximal pyloric response to leucine-enkephalin. We concluded 1) intraduodenal mixed amino acids or tryptophan increase phasic, spike-dependent pyloric contractions in the cat via nonvagal, naloxone-sensitive neural pathways, 2) phenylalanine, a structurally similar essential amino acid, had no effect on the feline gastroduodenal junction, and 3) the pyloric responses to exogenous CCK-OP are mediated by pathways distinct from the responses to tryptophan or mixed amino acids.",
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Opiate nerves mediate feline pyloric response to intraduodenal amino acids. / Reynolds, J. C.; Ouyang, Ann; Cohen, S.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 11, No. 3, 01.01.1985.

Research output: Contribution to journalArticle

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