Opiate Receptor‐Mediated Inhibition of Adenylate Cyclase in Rat Striatal Plasma Membranes

Dermot M.F. Cooper, Constantine Londos, Donald L. Gill, Martin Rodbell

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Abstract: Plasma membranes from rat striatum contain adenylate cyclase activity that is subject to dual regulation by GTP. Low concentrations (up to 30 nM) of the nucleotide increase activity whereas higher concentrations evoke a steady decline in activity; such behavior characterizes dually regulated adenylate cyclase systems. The opiates, morphine sulfate and D‐Ala‐Met‐enkephalin, produce naloxone‐reversible inhibition of the enzyme that is dependent on “inhibitory concentrations” of GTP (above 50 nM). In the absence of GTP no inhibition is observed. Sodium ions decrease the inhibition of activity promoted by GTP alone, but amplify the degree of inhibition seen in the presence of the opiates and GTP. The potencies of the opiates in mediating these effects mirror their affinities for 8 opiate receptors in striatum. It is suggested that this action of the opiates may represent their primary action in striatum.

Original languageEnglish (US)
Pages (from-to)1164-1167
Number of pages4
JournalJournal of neurochemistry
Volume38
Issue number4
DOIs
StatePublished - Apr 1982

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Opiate Alkaloids
Corpus Striatum
Cell membranes
Guanosine Triphosphate
Adenylyl Cyclases
Rats
Cell Membrane
Opioid Receptors
Morphine
Mirrors
Nucleotides
Sodium
Inhibition (Psychology)
Ions
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Cooper, Dermot M.F. ; Londos, Constantine ; Gill, Donald L. ; Rodbell, Martin. / Opiate Receptor‐Mediated Inhibition of Adenylate Cyclase in Rat Striatal Plasma Membranes. In: Journal of neurochemistry. 1982 ; Vol. 38, No. 4. pp. 1164-1167.
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Opiate Receptor‐Mediated Inhibition of Adenylate Cyclase in Rat Striatal Plasma Membranes. / Cooper, Dermot M.F.; Londos, Constantine; Gill, Donald L.; Rodbell, Martin.

In: Journal of neurochemistry, Vol. 38, No. 4, 04.1982, p. 1164-1167.

Research output: Contribution to journalArticle

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