Several detection techniques adapted for the capillary format have been applied to fluidic microchips, including optical, electrochemical, and mass spectrometric detection. While the aim of this chapter is not to review all of these various techniques, interested readers are directed to several recent discussions presented in the literature.1-6 Instead, the focus will be on optical methods, which will be described briefly along with some examples-the reader is also referred to the complimentary chapter on capillary-based systems by Sweedler in Chapter 9. Because of its broad application in microchip electrophoresis, a significant amount of discussion will be paid to laser-induced fluorescence (LIF) detection, which has played a key role in many of the microchip advancements achieved to date. This discussion will include a practical guide to assembling and aligning an LIF detection setup. Other optical techniques presented include ultraviolet (UV) absorption, chemiluminescence (CL) and electrochemiluminescence (ECL), Raman spectroscopy, thermal lens microscopy (TLM), surface plasmon resonance (SPR), and refractive index (RI) detection.
|Original language||English (US)|
|Title of host publication||Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition|
|Number of pages||24|
|State||Published - Jan 1 2007|
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Social Sciences(all)