Optical visualization and quantification of enzyme activity using dynamic droplet lenses

Lauren D. Zarzar, Julia A. Kalow, Xinping He, Joseph J. Walish, Timothy M. Swager

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In this paper, we describe an approach to measuring enzyme activity based on the reconfiguration of complex emulsions. Changes in the morphology of these complex emulsions, driven by enzyme-responsive surfactants, modulate the transmission of light through a sample. Through this method we demonstrate how simple photodetector measurements may be used to monitor enzyme kinetics. This approach is validated by quantitative measurements of enzyme activity for three different classes of enzymes (amylase, lipase, and sulfatase), relying on two distinct mechanisms for coupling droplet morphology to enzyme activity (host–guest interactions with uncaging and molecular cleavage).

Original languageEnglish (US)
Pages (from-to)3821-3825
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number15
DOIs
StatePublished - Apr 11 2017

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Lenses
Enzymes
Emulsions
Sulfatases
Amylases
Lipase
Surface-Active Agents
Light

All Science Journal Classification (ASJC) codes

  • General

Cite this

Zarzar, Lauren D. ; Kalow, Julia A. ; He, Xinping ; Walish, Joseph J. ; Swager, Timothy M. / Optical visualization and quantification of enzyme activity using dynamic droplet lenses. In: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Vol. 114, No. 15. pp. 3821-3825.
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Optical visualization and quantification of enzyme activity using dynamic droplet lenses. / Zarzar, Lauren D.; Kalow, Julia A.; He, Xinping; Walish, Joseph J.; Swager, Timothy M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 15, 11.04.2017, p. 3821-3825.

Research output: Contribution to journalArticle

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