Optimal heat-induced expression of the Drosophila hsp26 gene requires a promoter sequence containing (CT)n · (GA)n repeats

Robert L. Glaser, Claire Madison Thomas, Esther Siegfried, Sarah C R Elgin, John T. Lis

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

We report here the analysis of the sequence requirements for the heat-induced expression of the Drosophila melanogaster hsp26 gene using germline transformation. Heat-induced expression is augmented fivefold by a homopurine/homopyrimidine region from -85 to -134 that is devoid of heat-shock elements but contains numerous (dC-dT) · (dG-dA) repeats. Sequences within this interval have been shown to assume a nuclease S1-hypersensitive structure in vitro. In this paper, we extend those in vitro observations, demonstrating that the S1-hypersensitive structure is triple-helical H-DNA formed by a symmetric (dC-dT) · (dG-dA) sequence. Thus, the sequences that form H-DNA in vitro are also required in vivo for optimal hsp26 transcription. However, mutational analysis and diethylpyrocarbonate modification experiments in isolated nuclei suggest that the (dC-dT) · (dG-dA) sequence does not form H-DNA in vivo and argue against a role for H-DNA in the heat-induced expression of hsp26.

Original languageEnglish (US)
Pages (from-to)751-761
Number of pages11
JournalJournal of Molecular Biology
Volume211
Issue number4
DOIs
StatePublished - Feb 20 1990

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Drosophila
Hot Temperature
Genes
Diethyl Pyrocarbonate
Drosophila melanogaster
Sequence Analysis
Shock
poly(dG-dA)n.poly(dC-dT)n
triplex DNA
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Glaser, Robert L. ; Thomas, Claire Madison ; Siegfried, Esther ; Elgin, Sarah C R ; Lis, John T. / Optimal heat-induced expression of the Drosophila hsp26 gene requires a promoter sequence containing (CT)n · (GA)n repeats. In: Journal of Molecular Biology. 1990 ; Vol. 211, No. 4. pp. 751-761.
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Optimal heat-induced expression of the Drosophila hsp26 gene requires a promoter sequence containing (CT)n · (GA)n repeats. / Glaser, Robert L.; Thomas, Claire Madison; Siegfried, Esther; Elgin, Sarah C R; Lis, John T.

In: Journal of Molecular Biology, Vol. 211, No. 4, 20.02.1990, p. 751-761.

Research output: Contribution to journalArticle

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