Optimization of extracellular thermotolerant alkaline protease produced by marine Roseobacter sp. (MMD040)

S. Shanmughapriya, J. Krishnaveni, Joseph Selvin, R. Gandhimathi, M. Arunkumar, T. Thangavelu, G. Seghal Kiran, K. Natarajaseenivasan

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Abstract

Marine endosymbiontic Roseobacter sp. (MMD040), which produced high yields of protease, was isolated from marine sponge Fasciospongia cavernosa, collected from the peninsular coast of India. Maximum production of enzyme was obtained in Luria-Bertani broth. Catabolite repression was observed when the medium was supplemented with readily available carbon sources. The optimum temperature and pH for the enzyme production was 37°C and 7.0, respectively. The enzyme exhibited maximum activity in pH range of 6-9 with an optimum pH of 8.0 and retained nearly 92.5% activity at pH 9.0. The enzyme was stable at 40°C and showed 89% activity at 50°C. Based on the present findings, the enzyme was characterized as thermotolerant alkaline protease, which can be developed for industrial applications.

Original languageEnglish (US)
Pages (from-to)427-433
Number of pages7
JournalBioprocess and biosystems engineering
Volume31
Issue number5
DOIs
Publication statusPublished - Aug 1 2008

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering

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