Optimization of human lysozyme production by Kluyveromyces lactis K7 in biofilm reactor

Duygu Ercan, Ali Demirci

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Lysozyme (1,4-β-N-acetylmuramidase) is a lytic enzyme, which degrades a constituent of bacterial cell wall. Lysozyme has been of interest in medicine, cosmetics, and food industries because of its anti-bactericidal effect. Several approaches for the production of human lysozyme have been reported. Kluyveromyces lactis is a suitable host cell for the production of human lysozyme, because it has effective secretory capacity and crabtree negative effect in fully oxidative conditions. K. lactis K7 is a genetically modified organism that expresses human lysozyme. There is a need to improve the human lysozyme production by K. lactis K7 to make the human lysozyme more affordable. The aim of this study was to determine the best plastic composite support (PCS) and optimize the growth conditions of K. lactis K7 for the human lysozyme production in biofilm reactor with PCS. The best PCS type was selected according to the biofilm formation on PCS (CFU/g) and human lysozyme production (Unit/ml). After determination of the best PCS in test tubes, a bioreactor equipped with a 2 L vessel was implemented. To find the optimum combination of growth parameters, three factors Box-Behnken design was used and different temperatures (20-40°C), pH (4-7), and aeration levels (0-1.5 volume of air/volume of broth/minute) were tested. As a result, the optimum temperature for biomass production was found higher than the optimum temperature for lysozyme production. Moreover, while biomass was higher at pH 6, the lysozyme production increased as pH decreased. The aeration caused increase in biomass, but decrease in lysozyme production.

Original languageEnglish (US)
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2012, ASABE 2012
PublisherAmerican Society of Agricultural and Biological Engineers
Pages638-646
Number of pages9
ISBN (Print)9781622762088
StatePublished - Jan 1 2012
EventAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2012 - Dallas, TX, United States
Duration: Jul 29 2012Aug 1 2012

Publication series

NameAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2012, ASABE 2012
Volume1

Other

OtherAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2012
CountryUnited States
CityDallas, TX
Period7/29/128/1/12

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Bioengineering

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  • Cite this

    Ercan, D., & Demirci, A. (2012). Optimization of human lysozyme production by Kluyveromyces lactis K7 in biofilm reactor. In American Society of Agricultural and Biological Engineers Annual International Meeting 2012, ASABE 2012 (pp. 638-646). (American Society of Agricultural and Biological Engineers Annual International Meeting 2012, ASABE 2012; Vol. 1). American Society of Agricultural and Biological Engineers.