Optimization of PCR template concentrations for in vitro transcription-translation reactions

Lisa Renshaw-Gegg, Mark J. Guiltinan

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

In vitro transcription-translation reactions can be used to rapidly and inexpensively synthesize small amounts of protein from cloned DNA sequences for biochemical studies. The use of PCR to amplify template sequences has many advantageous over conventional protocols. However, the amount of template needs to be carefully adjusted for optimal production of protein products.

Original languageEnglish (US)
Pages (from-to)679-682
Number of pages4
JournalBiotechnology Letters
Volume18
Issue number6
DOIs
StatePublished - Jul 5 1996

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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