Lignocellulosic materials that are the most abundant plant biomass in the world have the potential to become sustainable sources of the produced value added products. Tea processing waste (TPW) is a good lignocellulosic source to produce the value added products from fermentable sugars. Therefore, the present study is undertaken to produce fermentable sugars by using ultrasound-assisted dilute acid hydrolysis of TPW followed by enzymatic hydrolysis. Furthermore, optimization of ultrasound-assisted dilute sulfuric acid (UADA) hydrolysis conditions of TPW was performed by response surface method at maximum power (900 W) for 2 h. Optimized conditions were 50°C, 1:6 (w/v) solid: liquid ratio, and 1%(w/v) dilute acid ratio, which yielded as 20.34 g/L fermentable sugar concentration. In conclusion, ultrasound-assisted dilute acid hydrolysis can be successfully applied for hydrolysis of TPW, which is a favorable carbon source for numerous value-added product productions via microbial fermentation.