Oral administration of astrovirus capsid protein is sufficient to induce acute diarrhea in vivo

Victoria A. Meliopoulos, Shauna A. Marvin, Pamela Freiden, Lindsey A. Moser, Prashant Nighot, Rizwana Ali, Anthony Blikslager, Muralidhar Reddivari, Richard J. Heath, Matthew D. Koci, Stacey Schultz-Cherry

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The disease mechanisms associated with the onset of astrovirus diarrhea are unknown. Unlike other enteric virus infections, astrovirus infection is not associated with an inflammatory response or cellular damage. In vitro studies in differentiated Caco-2 cells demonstrated that human astrovirus serotype 1 (HAstV-1) capsid protein alone disrupts the actin cytoskeleton and tight junction complex, leading to increased epithelial barrier permeability. In this study, we show that oral administration of purified recombinant turkey astrovirus 2 (TAstV-2) capsid protein results in acute diarrhea in a dose- and time-dependent manner in turkey poults. Similarly to that induced by infectious virus, TAstV-2 capsid-induced diarrhea was independent of inflammation or histological changes but was associated with increased intestinal barrier permeability, as well as redistribution of sodium hydrogen exchanger 3 (NHE3) from the membrane to the cytoplasm of the intestinal epithelium. Unlike other viral enterotoxins that have been identified, astrovirus capsid induces diarrhea after oral administration, reproducing the natural route of infection and demonstrating that ingestion of intact noninfectious capsid protein may be sufficient to provoke acute diarrhea. Based on these data, we hypothesize that the astrovirus capsid acts like an enterotoxin and induces intestinal epithelial barrier dysfunction. IMPORTANCE Acute gastroenteritis, with its sequela diarrhea, is one of the most important causes of childhood morbidity and mortality worldwide. A variety of infectious agents cause gastroenteritis, and in many cases, an enterotoxin produced by the agent is involved in disease manifestations. Although we commonly think of bacteria as a source of toxins, at least one enteric virus, rotavirus, produces a protein with enterotoxigenic activity during viral replication. In these studies, we demonstrate that oral administration of the turkey astrovirus 2 (TAstV-2) structural (capsid) protein induces acute diarrhea, increases barrier permeability, and causes relocalization of NHE3 in the small intestine, suggesting that rotavirus may not be alone in possessing enterotoxigenic activity.

Original languageEnglish (US)
Article numbere01494-16
JournalmBio
Volume7
Issue number6
DOIs
StatePublished - Jan 1 2016

Fingerprint

Capsid Proteins
Oral Administration
Diarrhea
Avastrovirus
Capsid
Enterotoxins
Permeability
Rotavirus
Gastroenteritis
Mamastrovirus
Enterovirus Infections
Caco-2 Cells
Enterovirus
Tight Junctions
Intestinal Mucosa
Infection
Actin Cytoskeleton
Small Intestine
Cytoplasm
Eating

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Virology

Cite this

Meliopoulos, V. A., Marvin, S. A., Freiden, P., Moser, L. A., Nighot, P., Ali, R., ... Schultz-Cherry, S. (2016). Oral administration of astrovirus capsid protein is sufficient to induce acute diarrhea in vivo. mBio, 7(6), [e01494-16]. https://doi.org/10.1128/mBio.01494-16
Meliopoulos, Victoria A. ; Marvin, Shauna A. ; Freiden, Pamela ; Moser, Lindsey A. ; Nighot, Prashant ; Ali, Rizwana ; Blikslager, Anthony ; Reddivari, Muralidhar ; Heath, Richard J. ; Koci, Matthew D. ; Schultz-Cherry, Stacey. / Oral administration of astrovirus capsid protein is sufficient to induce acute diarrhea in vivo. In: mBio. 2016 ; Vol. 7, No. 6.
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Meliopoulos, VA, Marvin, SA, Freiden, P, Moser, LA, Nighot, P, Ali, R, Blikslager, A, Reddivari, M, Heath, RJ, Koci, MD & Schultz-Cherry, S 2016, 'Oral administration of astrovirus capsid protein is sufficient to induce acute diarrhea in vivo', mBio, vol. 7, no. 6, e01494-16. https://doi.org/10.1128/mBio.01494-16

Oral administration of astrovirus capsid protein is sufficient to induce acute diarrhea in vivo. / Meliopoulos, Victoria A.; Marvin, Shauna A.; Freiden, Pamela; Moser, Lindsey A.; Nighot, Prashant; Ali, Rizwana; Blikslager, Anthony; Reddivari, Muralidhar; Heath, Richard J.; Koci, Matthew D.; Schultz-Cherry, Stacey.

In: mBio, Vol. 7, No. 6, e01494-16, 01.01.2016.

Research output: Contribution to journalArticle

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AU - Meliopoulos, Victoria A.

AU - Marvin, Shauna A.

AU - Freiden, Pamela

AU - Moser, Lindsey A.

AU - Nighot, Prashant

AU - Ali, Rizwana

AU - Blikslager, Anthony

AU - Reddivari, Muralidhar

AU - Heath, Richard J.

AU - Koci, Matthew D.

AU - Schultz-Cherry, Stacey

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N2 - The disease mechanisms associated with the onset of astrovirus diarrhea are unknown. Unlike other enteric virus infections, astrovirus infection is not associated with an inflammatory response or cellular damage. In vitro studies in differentiated Caco-2 cells demonstrated that human astrovirus serotype 1 (HAstV-1) capsid protein alone disrupts the actin cytoskeleton and tight junction complex, leading to increased epithelial barrier permeability. In this study, we show that oral administration of purified recombinant turkey astrovirus 2 (TAstV-2) capsid protein results in acute diarrhea in a dose- and time-dependent manner in turkey poults. Similarly to that induced by infectious virus, TAstV-2 capsid-induced diarrhea was independent of inflammation or histological changes but was associated with increased intestinal barrier permeability, as well as redistribution of sodium hydrogen exchanger 3 (NHE3) from the membrane to the cytoplasm of the intestinal epithelium. Unlike other viral enterotoxins that have been identified, astrovirus capsid induces diarrhea after oral administration, reproducing the natural route of infection and demonstrating that ingestion of intact noninfectious capsid protein may be sufficient to provoke acute diarrhea. Based on these data, we hypothesize that the astrovirus capsid acts like an enterotoxin and induces intestinal epithelial barrier dysfunction. IMPORTANCE Acute gastroenteritis, with its sequela diarrhea, is one of the most important causes of childhood morbidity and mortality worldwide. A variety of infectious agents cause gastroenteritis, and in many cases, an enterotoxin produced by the agent is involved in disease manifestations. Although we commonly think of bacteria as a source of toxins, at least one enteric virus, rotavirus, produces a protein with enterotoxigenic activity during viral replication. In these studies, we demonstrate that oral administration of the turkey astrovirus 2 (TAstV-2) structural (capsid) protein induces acute diarrhea, increases barrier permeability, and causes relocalization of NHE3 in the small intestine, suggesting that rotavirus may not be alone in possessing enterotoxigenic activity.

AB - The disease mechanisms associated with the onset of astrovirus diarrhea are unknown. Unlike other enteric virus infections, astrovirus infection is not associated with an inflammatory response or cellular damage. In vitro studies in differentiated Caco-2 cells demonstrated that human astrovirus serotype 1 (HAstV-1) capsid protein alone disrupts the actin cytoskeleton and tight junction complex, leading to increased epithelial barrier permeability. In this study, we show that oral administration of purified recombinant turkey astrovirus 2 (TAstV-2) capsid protein results in acute diarrhea in a dose- and time-dependent manner in turkey poults. Similarly to that induced by infectious virus, TAstV-2 capsid-induced diarrhea was independent of inflammation or histological changes but was associated with increased intestinal barrier permeability, as well as redistribution of sodium hydrogen exchanger 3 (NHE3) from the membrane to the cytoplasm of the intestinal epithelium. Unlike other viral enterotoxins that have been identified, astrovirus capsid induces diarrhea after oral administration, reproducing the natural route of infection and demonstrating that ingestion of intact noninfectious capsid protein may be sufficient to provoke acute diarrhea. Based on these data, we hypothesize that the astrovirus capsid acts like an enterotoxin and induces intestinal epithelial barrier dysfunction. IMPORTANCE Acute gastroenteritis, with its sequela diarrhea, is one of the most important causes of childhood morbidity and mortality worldwide. A variety of infectious agents cause gastroenteritis, and in many cases, an enterotoxin produced by the agent is involved in disease manifestations. Although we commonly think of bacteria as a source of toxins, at least one enteric virus, rotavirus, produces a protein with enterotoxigenic activity during viral replication. In these studies, we demonstrate that oral administration of the turkey astrovirus 2 (TAstV-2) structural (capsid) protein induces acute diarrhea, increases barrier permeability, and causes relocalization of NHE3 in the small intestine, suggesting that rotavirus may not be alone in possessing enterotoxigenic activity.

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