Organ culture of human endometrium. Effect of ovarian steroids

Edward C. Hughes, Laurence Demers, Tiiu Csermely, David B. Jones

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Glycogen turnover was determined in 86 normal and 52 sterility patients through the chemical analysis of endometrial glycogen content, glycogen synthetase and glycogen phosphorylase activities. Specimens from 38 of these normal patients as well as 31 sterility patients were also subjected to the organ culture process. These studies suggest to us that (1) an organ culture system is an ideal avenue of approach for the study of this glandular epithelial tissue and (2) that estradiol is not solely responsible for the increase in glycogen deposition in human endometrium, but that progesterone following estrogen priming is essential for a complete response of glycogenesis. This study further demonstrates in organ culture that estradiol-induced proliferative changes in the tissue, while progesterone not only caused cellular alterations but also induced an increase in glycogen deposition. Preliminary studies with the enzyme systems in organ culture suggest a role of progesterone in phosphorylase activity, particularly during the secretory phase where a controlled breakdown of glycogen is desired.

Original languageEnglish (US)
Pages (from-to)707-720
Number of pages14
JournalAmerican journal of obstetrics and gynecology
Volume105
Issue number5
DOIs
StatePublished - Nov 1 1969

Fingerprint

Organ Culture Techniques
Endometrium
Glycogen
Steroids
Progesterone
Infertility
Estradiol
Glycogen Phosphorylase
Glycogen Synthase
Phosphorylases
Estrogens
Epithelium
Enzymes

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynecology

Cite this

Hughes, Edward C. ; Demers, Laurence ; Csermely, Tiiu ; Jones, David B. / Organ culture of human endometrium. Effect of ovarian steroids. In: American journal of obstetrics and gynecology. 1969 ; Vol. 105, No. 5. pp. 707-720.
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Organ culture of human endometrium. Effect of ovarian steroids. / Hughes, Edward C.; Demers, Laurence; Csermely, Tiiu; Jones, David B.

In: American journal of obstetrics and gynecology, Vol. 105, No. 5, 01.11.1969, p. 707-720.

Research output: Contribution to journalArticle

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