Organization and expression analysis of the zebrafish hepcidin gene, an antimicrobial peptide gene conserved among vertebrates

Hiroko Shike, Chisato Shimizu, Xavier Lauth, Jane C. Burns

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Hepcidin is an antimicrobial peptide and iron-regulatory molecule that is conserved among vertebrates. Mutations or over-expression of the human hepcidin gene have been found in patients with hemochromatosis and refractory anemia. To further understand the function and regulation of hepcidin, animal models are needed. We sequenced cDNA, genes and upstream regions of zebrafish hepcidin and analyzed gene expression by kinetic PCR. Zebrafish hepcidin genes consist of two introns and three exons that encode a prepropeptide (91 amino acids). The amino acid sequences and gene organization were remarkably conserved between zebrafish and other species. Elevated gene expression was observed in abdominal organs, skin, and heart in fish that developed signs of infection following bacterial injection. Zebrafish may be a suitable model organism for further study of hepcidin gene regulation.

Original languageEnglish (US)
Pages (from-to)747-754
Number of pages8
JournalDevelopmental and Comparative Immunology
Volume28
Issue number7-8
DOIs
StatePublished - Jun 1 2004

Fingerprint

Hepcidins
Zebrafish
Vertebrates
Peptides
Genes
Refractory Anemia
Gene Expression
Hemochromatosis
Bacterial Infections
Introns
Amino Acid Sequence
Exons
Fishes
Iron
Animal Models
Complementary DNA
Amino Acids
Polymerase Chain Reaction
Skin
Mutation

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

Cite this

@article{3f935bc735c84024a962cd49cdb4fb89,
title = "Organization and expression analysis of the zebrafish hepcidin gene, an antimicrobial peptide gene conserved among vertebrates",
abstract = "Hepcidin is an antimicrobial peptide and iron-regulatory molecule that is conserved among vertebrates. Mutations or over-expression of the human hepcidin gene have been found in patients with hemochromatosis and refractory anemia. To further understand the function and regulation of hepcidin, animal models are needed. We sequenced cDNA, genes and upstream regions of zebrafish hepcidin and analyzed gene expression by kinetic PCR. Zebrafish hepcidin genes consist of two introns and three exons that encode a prepropeptide (91 amino acids). The amino acid sequences and gene organization were remarkably conserved between zebrafish and other species. Elevated gene expression was observed in abdominal organs, skin, and heart in fish that developed signs of infection following bacterial injection. Zebrafish may be a suitable model organism for further study of hepcidin gene regulation.",
author = "Hiroko Shike and Chisato Shimizu and Xavier Lauth and Burns, {Jane C.}",
year = "2004",
month = "6",
day = "1",
doi = "10.1016/j.dci.2003.11.009",
language = "English (US)",
volume = "28",
pages = "747--754",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Elsevier Limited",
number = "7-8",

}

Organization and expression analysis of the zebrafish hepcidin gene, an antimicrobial peptide gene conserved among vertebrates. / Shike, Hiroko; Shimizu, Chisato; Lauth, Xavier; Burns, Jane C.

In: Developmental and Comparative Immunology, Vol. 28, No. 7-8, 01.06.2004, p. 747-754.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Organization and expression analysis of the zebrafish hepcidin gene, an antimicrobial peptide gene conserved among vertebrates

AU - Shike, Hiroko

AU - Shimizu, Chisato

AU - Lauth, Xavier

AU - Burns, Jane C.

PY - 2004/6/1

Y1 - 2004/6/1

N2 - Hepcidin is an antimicrobial peptide and iron-regulatory molecule that is conserved among vertebrates. Mutations or over-expression of the human hepcidin gene have been found in patients with hemochromatosis and refractory anemia. To further understand the function and regulation of hepcidin, animal models are needed. We sequenced cDNA, genes and upstream regions of zebrafish hepcidin and analyzed gene expression by kinetic PCR. Zebrafish hepcidin genes consist of two introns and three exons that encode a prepropeptide (91 amino acids). The amino acid sequences and gene organization were remarkably conserved between zebrafish and other species. Elevated gene expression was observed in abdominal organs, skin, and heart in fish that developed signs of infection following bacterial injection. Zebrafish may be a suitable model organism for further study of hepcidin gene regulation.

AB - Hepcidin is an antimicrobial peptide and iron-regulatory molecule that is conserved among vertebrates. Mutations or over-expression of the human hepcidin gene have been found in patients with hemochromatosis and refractory anemia. To further understand the function and regulation of hepcidin, animal models are needed. We sequenced cDNA, genes and upstream regions of zebrafish hepcidin and analyzed gene expression by kinetic PCR. Zebrafish hepcidin genes consist of two introns and three exons that encode a prepropeptide (91 amino acids). The amino acid sequences and gene organization were remarkably conserved between zebrafish and other species. Elevated gene expression was observed in abdominal organs, skin, and heart in fish that developed signs of infection following bacterial injection. Zebrafish may be a suitable model organism for further study of hepcidin gene regulation.

UR - http://www.scopus.com/inward/record.url?scp=1642386686&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642386686&partnerID=8YFLogxK

U2 - 10.1016/j.dci.2003.11.009

DO - 10.1016/j.dci.2003.11.009

M3 - Article

C2 - 15043943

AN - SCOPUS:1642386686

VL - 28

SP - 747

EP - 754

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

IS - 7-8

ER -