Organization of mhc class ii b genes in the zebrafish (brachydanio rerio)

Holger Sültmann, Werner E. Mayer, Figueroa Figueroa, Colm O’Huigin, Jan Klein

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Using three genomic phage libraries, we isolated 26 clones from the zebrafish MHC class II B region. By restriction mapping, the clones could be arranged into six clusters, most clusters consisting of several overlapping clones. The combined clusters cover a total of 161 kb of the zebrafish class II region. Hybridization with specific probes demonstrated the presence in the clusters of two class II A and six class II B genes. Sequencing of the B genes revealed that they represented six different families of class II loci. Only two of the class II B and one of the class II A genes are complete; the others are truncated pseudogenes. Only one of the class II B loci shows extensive restriction fragment length polymorphism. This is also the only locus found to be transcribed in organs with large numbers of lymphoid or myeloid cells. The zebrafish class II genes have promoter regions with sequence elements found previously in mammalian genes and known to be involved in regulation of expression. The exon-intron organization of the zebrafish class II genes is similar to that of the mammalian genes, but the introns are characteristically short, ranging in length from 74 to 362 bp. The distances between A and B genes in a given pair are also short, but the distances between B genes are as long as or longer than those between mammalian class II B genes. All of the zebrafish class II B genes appear to have arisen by duplication and diversification of a single ancestral B gene after the separation of bony fishes from other vertebrate taxa.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalGenomics
Volume23
Issue number1
DOIs
StatePublished - Sep 1994

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Zebrafish
MHC Class II Genes
Genes
Clone Cells
Introns
vif Genes
Restriction Mapping
Pseudogenes
Genomic Library
Myeloid Cells
Genetic Promoter Regions
Restriction Fragment Length Polymorphisms
Bacteriophages
Vertebrates
Exons
Fishes
Lymphocytes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Sültmann, H., Mayer, W. E., Figueroa, F., O’Huigin, C., & Klein, J. (1994). Organization of mhc class ii b genes in the zebrafish (brachydanio rerio). Genomics, 23(1), 1-14. https://doi.org/10.1006/geno.1994.1452
Sültmann, Holger ; Mayer, Werner E. ; Figueroa, Figueroa ; O’Huigin, Colm ; Klein, Jan. / Organization of mhc class ii b genes in the zebrafish (brachydanio rerio). In: Genomics. 1994 ; Vol. 23, No. 1. pp. 1-14.
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Sültmann, H, Mayer, WE, Figueroa, F, O’Huigin, C & Klein, J 1994, 'Organization of mhc class ii b genes in the zebrafish (brachydanio rerio)', Genomics, vol. 23, no. 1, pp. 1-14. https://doi.org/10.1006/geno.1994.1452

Organization of mhc class ii b genes in the zebrafish (brachydanio rerio). / Sültmann, Holger; Mayer, Werner E.; Figueroa, Figueroa; O’Huigin, Colm; Klein, Jan.

In: Genomics, Vol. 23, No. 1, 09.1994, p. 1-14.

Research output: Contribution to journalArticle

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Sültmann H, Mayer WE, Figueroa F, O’Huigin C, Klein J. Organization of mhc class ii b genes in the zebrafish (brachydanio rerio). Genomics. 1994 Sep;23(1):1-14. https://doi.org/10.1006/geno.1994.1452