TY - JOUR
T1 - Organization of the human SP-A and SP-D loci at 10q22-q23 physical and radiation hybrid mapping reveal gene order and orientation
AU - Hoover, Russell R.
AU - Floros, Joanna
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1998
Y1 - 1998
N2 - The human surfactant protein (SP) A locus has been assigned to chromosome 10q22-q23 and consists of two very similar genes, SP-A1 and SP-A2, as well as a truncated pseudogene. SP-A belongs to the family of collagenous C-type lectins along with mannose binding protein (MBP) and SP-D, both of which have also been mapped to the long arm of chromosome 10. In this article we report the relative location and orientation of each of the SP-A and SP-D genomic sequences. Characterization of two overlapping genomic clones revealed that the SP-A pseudogene lies in a reverse orientation 15 kb away from the 5′ side of SP-Al. This finding was verified by the amplification of the entire SP-A pseudogene/SP-Al intergenic region using long-range polymerase chain reaction. The relative location of SP-A2 and SP-D was then ascertained by testing a number of sequence tagged sites against the Stanford TNG3 and G3 radiation hybrid panels. The radiation hybrid mapping data showed that both SP-A2 and SP-D are on the 5′ side of SP-Al at approximate distances of 40 kb and 120 kb, respectively. The SP-A and SP-D loci were also oriented relative to the centromere, with the overall order being: centromere - SP-D - SP-A2 - pseudogene - SP-Al -telomere.
AB - The human surfactant protein (SP) A locus has been assigned to chromosome 10q22-q23 and consists of two very similar genes, SP-A1 and SP-A2, as well as a truncated pseudogene. SP-A belongs to the family of collagenous C-type lectins along with mannose binding protein (MBP) and SP-D, both of which have also been mapped to the long arm of chromosome 10. In this article we report the relative location and orientation of each of the SP-A and SP-D genomic sequences. Characterization of two overlapping genomic clones revealed that the SP-A pseudogene lies in a reverse orientation 15 kb away from the 5′ side of SP-Al. This finding was verified by the amplification of the entire SP-A pseudogene/SP-Al intergenic region using long-range polymerase chain reaction. The relative location of SP-A2 and SP-D was then ascertained by testing a number of sequence tagged sites against the Stanford TNG3 and G3 radiation hybrid panels. The radiation hybrid mapping data showed that both SP-A2 and SP-D are on the 5′ side of SP-Al at approximate distances of 40 kb and 120 kb, respectively. The SP-A and SP-D loci were also oriented relative to the centromere, with the overall order being: centromere - SP-D - SP-A2 - pseudogene - SP-Al -telomere.
UR - http://www.scopus.com/inward/record.url?scp=0032015118&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032015118&partnerID=8YFLogxK
U2 - 10.1165/ajrcmb.18.3.3035
DO - 10.1165/ajrcmb.18.3.3035
M3 - Article
C2 - 9490653
AN - SCOPUS:0032015118
VL - 18
SP - 353
EP - 362
JO - American Journal of Respiratory Cell and Molecular Biology
JF - American Journal of Respiratory Cell and Molecular Biology
SN - 1044-1549
IS - 3
ER -