Oriented Adhesion of Escherichia coli to Polystyrene Particles

Joseph F. Jones, Jason D. Feick, Daniel Imoudu, Nkiru Chukwumah, Margot Vigeant, Darrell Velegol

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The adhesion of nonflagellated Escherichia coli strain K-12 to polystyrene (PS) latex spheres or glass capillaries has been observed by using several techniques. Attention was focused on the orientation of the rod-shaped bacteria as they adhered to the surfaces in 100 mM phosphate-buffered saline. Data show that PS particles adhered to the ends of the bacteria more than 90% of the time. Moreover, the PS particles adhered to one end only, never to both. Similarly, for experiments with bacteria adhering to glass, the bacteria adhered on their ends. In order to determine whether the end of a bacterium had a different charge density from that of the middle, rotational electrophoresis experiments were used. These experiments indicated no measurable charge nonuniformity. In order to examine how strongly adhered the bacteria were to the PS particles, differential electrophoresis was used. Almost always, bacteria were found to be irreversibly adhered to the PS spheres. The cause of the oriented adhesion is not likely due to surface lipopolysaccharides (LPS), since the three strains of K-12 that were used, each having a different length of LPS, showed similar behavior. The results are discussed in terms of bacterial cell polarity. The data indicate that nanodomains on the bacterial ends are important for adhesion and that the time scale for irreversible adhesion is short.

Original languageEnglish (US)
Pages (from-to)6515-6519
Number of pages5
JournalApplied and environmental microbiology
Volume69
Issue number11
DOIs
StatePublished - Nov 1 2003

Fingerprint

Polystyrenes
polystyrenes
adhesion
Escherichia coli
Bacteria
bacterium
bacteria
lipopolysaccharides
Glass
electrophoresis
Lipopolysaccharides
Electrophoresis
glass
electrokinesis
Cell Polarity
experiment
latex
particle
Microspheres
Phosphates

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Jones, Joseph F. ; Feick, Jason D. ; Imoudu, Daniel ; Chukwumah, Nkiru ; Vigeant, Margot ; Velegol, Darrell. / Oriented Adhesion of Escherichia coli to Polystyrene Particles. In: Applied and environmental microbiology. 2003 ; Vol. 69, No. 11. pp. 6515-6519.
@article{fc2792478cf244b7bff87db44a8da157,
title = "Oriented Adhesion of Escherichia coli to Polystyrene Particles",
abstract = "The adhesion of nonflagellated Escherichia coli strain K-12 to polystyrene (PS) latex spheres or glass capillaries has been observed by using several techniques. Attention was focused on the orientation of the rod-shaped bacteria as they adhered to the surfaces in 100 mM phosphate-buffered saline. Data show that PS particles adhered to the ends of the bacteria more than 90{\%} of the time. Moreover, the PS particles adhered to one end only, never to both. Similarly, for experiments with bacteria adhering to glass, the bacteria adhered on their ends. In order to determine whether the end of a bacterium had a different charge density from that of the middle, rotational electrophoresis experiments were used. These experiments indicated no measurable charge nonuniformity. In order to examine how strongly adhered the bacteria were to the PS particles, differential electrophoresis was used. Almost always, bacteria were found to be irreversibly adhered to the PS spheres. The cause of the oriented adhesion is not likely due to surface lipopolysaccharides (LPS), since the three strains of K-12 that were used, each having a different length of LPS, showed similar behavior. The results are discussed in terms of bacterial cell polarity. The data indicate that nanodomains on the bacterial ends are important for adhesion and that the time scale for irreversible adhesion is short.",
author = "Jones, {Joseph F.} and Feick, {Jason D.} and Daniel Imoudu and Nkiru Chukwumah and Margot Vigeant and Darrell Velegol",
year = "2003",
month = "11",
day = "1",
doi = "10.1128/AEM.69.11.6515-6519.2003",
language = "English (US)",
volume = "69",
pages = "6515--6519",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "11",

}

Oriented Adhesion of Escherichia coli to Polystyrene Particles. / Jones, Joseph F.; Feick, Jason D.; Imoudu, Daniel; Chukwumah, Nkiru; Vigeant, Margot; Velegol, Darrell.

In: Applied and environmental microbiology, Vol. 69, No. 11, 01.11.2003, p. 6515-6519.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Oriented Adhesion of Escherichia coli to Polystyrene Particles

AU - Jones, Joseph F.

AU - Feick, Jason D.

AU - Imoudu, Daniel

AU - Chukwumah, Nkiru

AU - Vigeant, Margot

AU - Velegol, Darrell

PY - 2003/11/1

Y1 - 2003/11/1

N2 - The adhesion of nonflagellated Escherichia coli strain K-12 to polystyrene (PS) latex spheres or glass capillaries has been observed by using several techniques. Attention was focused on the orientation of the rod-shaped bacteria as they adhered to the surfaces in 100 mM phosphate-buffered saline. Data show that PS particles adhered to the ends of the bacteria more than 90% of the time. Moreover, the PS particles adhered to one end only, never to both. Similarly, for experiments with bacteria adhering to glass, the bacteria adhered on their ends. In order to determine whether the end of a bacterium had a different charge density from that of the middle, rotational electrophoresis experiments were used. These experiments indicated no measurable charge nonuniformity. In order to examine how strongly adhered the bacteria were to the PS particles, differential electrophoresis was used. Almost always, bacteria were found to be irreversibly adhered to the PS spheres. The cause of the oriented adhesion is not likely due to surface lipopolysaccharides (LPS), since the three strains of K-12 that were used, each having a different length of LPS, showed similar behavior. The results are discussed in terms of bacterial cell polarity. The data indicate that nanodomains on the bacterial ends are important for adhesion and that the time scale for irreversible adhesion is short.

AB - The adhesion of nonflagellated Escherichia coli strain K-12 to polystyrene (PS) latex spheres or glass capillaries has been observed by using several techniques. Attention was focused on the orientation of the rod-shaped bacteria as they adhered to the surfaces in 100 mM phosphate-buffered saline. Data show that PS particles adhered to the ends of the bacteria more than 90% of the time. Moreover, the PS particles adhered to one end only, never to both. Similarly, for experiments with bacteria adhering to glass, the bacteria adhered on their ends. In order to determine whether the end of a bacterium had a different charge density from that of the middle, rotational electrophoresis experiments were used. These experiments indicated no measurable charge nonuniformity. In order to examine how strongly adhered the bacteria were to the PS particles, differential electrophoresis was used. Almost always, bacteria were found to be irreversibly adhered to the PS spheres. The cause of the oriented adhesion is not likely due to surface lipopolysaccharides (LPS), since the three strains of K-12 that were used, each having a different length of LPS, showed similar behavior. The results are discussed in terms of bacterial cell polarity. The data indicate that nanodomains on the bacterial ends are important for adhesion and that the time scale for irreversible adhesion is short.

UR - http://www.scopus.com/inward/record.url?scp=0242405817&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242405817&partnerID=8YFLogxK

U2 - 10.1128/AEM.69.11.6515-6519.2003

DO - 10.1128/AEM.69.11.6515-6519.2003

M3 - Article

VL - 69

SP - 6515

EP - 6519

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 11

ER -