Origin and interstate spread of a New York City multidrug-resistant Mycobacterium tuberculosis clone family

Pablo J. Bifani, Bonnie B. Plikaytis, Vivek Kapur, Kathryn Stockbauer, Xi Pan, Michael L. Lutfey, Soraya L. Moghazeh, William Eisner, Thomas M. Daniel, Mark H. Kaplan, Jack T. Crawford, James M. Musser, Barry N. Kreiswirth

Research output: Contribution to journalArticle

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Abstract

Objective. - To determine whether isolates of Mycobacterium tuberculosis from New York and elsewhere that are resistant to four or more primary antimicrobial agents and responsible for widespread disease in the 1990s represent a newly emerged clone or a heterogeneous array of unrelated organisms. Setting. - New York City area and selected locations in the United States. Patients. - M tuberculosis isolates from 1953 patients in New York and multidrug-resistant isolates from six patients from other US communities. Design. - Convenience sample of all M tuberculosis strains (M tuberculosis isolates resistant to rifampin, streptomycin, isoniazid, and ethambutol, and sometimes ethionamide, kanamycin, capreomycin, or ciprofloxacin) submitted to the Public Health Research Institute Tuberculosis Center since 1991 and samples submitted to the Centers for Disease Control and Prevention from throughout the United States. The samples submitted were representative of the New York City strains of M tuberculosis. Main Outcome Measure. - Characterization of resistant M tuberculosis strains studied by IS6110 and polymorphic GC-rich repetitive sequence (PGRS) hybridization patterns, multiplex polymerase chain reaction (PCR) analysis, and automated DNA sequencing of genes containing mutations associated with resistance to rifampin (rpoB), isoniazid (katG and inhA locus), and streptomycin (strA and rrs). Results. - Multidrug-resistant M tuberculosis isolates were recovered from 253 New York City patients and had the same or closely allied IS6110 and PGRS patterns, multiplex PCR type, and gene mutations associated with resistance to rifampin, isoniazid, and streptomycin. Isolates with these same molecular characteristics were recovered from patients in Florida and Nevada, health care workers in Atlanta, Ga, and Miami, Fla, and an individual who recently moved from New York City to Denver, Colo, and caused disease or skin test conversion in at least 12 people in a nursing home environment. Conclusions. - The results document the molecular origin and spread of progeny of a closely related family of multidrug-resistant M tuberculosis strains that have recently shared a common ancestor and undergone clonal expansion. The multidrug-resistant phenotype in these organisms arose by sequential acquisition of resistance-conferring mutations in several genes, most likely as a consequence of antibiotic selection of randomly occurring mutants in concert with inadequately treated infections. Dissemination of these difficult-to-treat bacteria throughout New York City and to at least four additional US cities has adverse implications for tuberculosis control in the 21st century.

Original languageEnglish (US)
Pages (from-to)452-457
Number of pages6
JournalJournal of the American Medical Association
Volume275
Issue number6
DOIs
StatePublished - Feb 14 1996

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Multidrug-Resistant Tuberculosis
Mycobacterium tuberculosis
Tuberculosis
Clone Cells
Isoniazid
Streptomycin
Rifampin
GC Rich Sequence
Nucleic Acid Repetitive Sequences
Multiplex Polymerase Chain Reaction
Mutation
Capreomycin
Ethionamide
Genes
Ethambutol
Kanamycin
Centers for Disease Control and Prevention (U.S.)
Ciprofloxacin
Anti-Infective Agents
Nursing Homes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Bifani, Pablo J. ; Plikaytis, Bonnie B. ; Kapur, Vivek ; Stockbauer, Kathryn ; Pan, Xi ; Lutfey, Michael L. ; Moghazeh, Soraya L. ; Eisner, William ; Daniel, Thomas M. ; Kaplan, Mark H. ; Crawford, Jack T. ; Musser, James M. ; Kreiswirth, Barry N. / Origin and interstate spread of a New York City multidrug-resistant Mycobacterium tuberculosis clone family. In: Journal of the American Medical Association. 1996 ; Vol. 275, No. 6. pp. 452-457.
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abstract = "Objective. - To determine whether isolates of Mycobacterium tuberculosis from New York and elsewhere that are resistant to four or more primary antimicrobial agents and responsible for widespread disease in the 1990s represent a newly emerged clone or a heterogeneous array of unrelated organisms. Setting. - New York City area and selected locations in the United States. Patients. - M tuberculosis isolates from 1953 patients in New York and multidrug-resistant isolates from six patients from other US communities. Design. - Convenience sample of all M tuberculosis strains (M tuberculosis isolates resistant to rifampin, streptomycin, isoniazid, and ethambutol, and sometimes ethionamide, kanamycin, capreomycin, or ciprofloxacin) submitted to the Public Health Research Institute Tuberculosis Center since 1991 and samples submitted to the Centers for Disease Control and Prevention from throughout the United States. The samples submitted were representative of the New York City strains of M tuberculosis. Main Outcome Measure. - Characterization of resistant M tuberculosis strains studied by IS6110 and polymorphic GC-rich repetitive sequence (PGRS) hybridization patterns, multiplex polymerase chain reaction (PCR) analysis, and automated DNA sequencing of genes containing mutations associated with resistance to rifampin (rpoB), isoniazid (katG and inhA locus), and streptomycin (strA and rrs). Results. - Multidrug-resistant M tuberculosis isolates were recovered from 253 New York City patients and had the same or closely allied IS6110 and PGRS patterns, multiplex PCR type, and gene mutations associated with resistance to rifampin, isoniazid, and streptomycin. Isolates with these same molecular characteristics were recovered from patients in Florida and Nevada, health care workers in Atlanta, Ga, and Miami, Fla, and an individual who recently moved from New York City to Denver, Colo, and caused disease or skin test conversion in at least 12 people in a nursing home environment. Conclusions. - The results document the molecular origin and spread of progeny of a closely related family of multidrug-resistant M tuberculosis strains that have recently shared a common ancestor and undergone clonal expansion. The multidrug-resistant phenotype in these organisms arose by sequential acquisition of resistance-conferring mutations in several genes, most likely as a consequence of antibiotic selection of randomly occurring mutants in concert with inadequately treated infections. Dissemination of these difficult-to-treat bacteria throughout New York City and to at least four additional US cities has adverse implications for tuberculosis control in the 21st century.",
author = "Bifani, {Pablo J.} and Plikaytis, {Bonnie B.} and Vivek Kapur and Kathryn Stockbauer and Xi Pan and Lutfey, {Michael L.} and Moghazeh, {Soraya L.} and William Eisner and Daniel, {Thomas M.} and Kaplan, {Mark H.} and Crawford, {Jack T.} and Musser, {James M.} and Kreiswirth, {Barry N.}",
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Bifani, PJ, Plikaytis, BB, Kapur, V, Stockbauer, K, Pan, X, Lutfey, ML, Moghazeh, SL, Eisner, W, Daniel, TM, Kaplan, MH, Crawford, JT, Musser, JM & Kreiswirth, BN 1996, 'Origin and interstate spread of a New York City multidrug-resistant Mycobacterium tuberculosis clone family', Journal of the American Medical Association, vol. 275, no. 6, pp. 452-457. https://doi.org/10.1001/jama.275.6.452

Origin and interstate spread of a New York City multidrug-resistant Mycobacterium tuberculosis clone family. / Bifani, Pablo J.; Plikaytis, Bonnie B.; Kapur, Vivek; Stockbauer, Kathryn; Pan, Xi; Lutfey, Michael L.; Moghazeh, Soraya L.; Eisner, William; Daniel, Thomas M.; Kaplan, Mark H.; Crawford, Jack T.; Musser, James M.; Kreiswirth, Barry N.

In: Journal of the American Medical Association, Vol. 275, No. 6, 14.02.1996, p. 452-457.

Research output: Contribution to journalArticle

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T1 - Origin and interstate spread of a New York City multidrug-resistant Mycobacterium tuberculosis clone family

AU - Bifani, Pablo J.

AU - Plikaytis, Bonnie B.

AU - Kapur, Vivek

AU - Stockbauer, Kathryn

AU - Pan, Xi

AU - Lutfey, Michael L.

AU - Moghazeh, Soraya L.

AU - Eisner, William

AU - Daniel, Thomas M.

AU - Kaplan, Mark H.

AU - Crawford, Jack T.

AU - Musser, James M.

AU - Kreiswirth, Barry N.

PY - 1996/2/14

Y1 - 1996/2/14

N2 - Objective. - To determine whether isolates of Mycobacterium tuberculosis from New York and elsewhere that are resistant to four or more primary antimicrobial agents and responsible for widespread disease in the 1990s represent a newly emerged clone or a heterogeneous array of unrelated organisms. Setting. - New York City area and selected locations in the United States. Patients. - M tuberculosis isolates from 1953 patients in New York and multidrug-resistant isolates from six patients from other US communities. Design. - Convenience sample of all M tuberculosis strains (M tuberculosis isolates resistant to rifampin, streptomycin, isoniazid, and ethambutol, and sometimes ethionamide, kanamycin, capreomycin, or ciprofloxacin) submitted to the Public Health Research Institute Tuberculosis Center since 1991 and samples submitted to the Centers for Disease Control and Prevention from throughout the United States. The samples submitted were representative of the New York City strains of M tuberculosis. Main Outcome Measure. - Characterization of resistant M tuberculosis strains studied by IS6110 and polymorphic GC-rich repetitive sequence (PGRS) hybridization patterns, multiplex polymerase chain reaction (PCR) analysis, and automated DNA sequencing of genes containing mutations associated with resistance to rifampin (rpoB), isoniazid (katG and inhA locus), and streptomycin (strA and rrs). Results. - Multidrug-resistant M tuberculosis isolates were recovered from 253 New York City patients and had the same or closely allied IS6110 and PGRS patterns, multiplex PCR type, and gene mutations associated with resistance to rifampin, isoniazid, and streptomycin. Isolates with these same molecular characteristics were recovered from patients in Florida and Nevada, health care workers in Atlanta, Ga, and Miami, Fla, and an individual who recently moved from New York City to Denver, Colo, and caused disease or skin test conversion in at least 12 people in a nursing home environment. Conclusions. - The results document the molecular origin and spread of progeny of a closely related family of multidrug-resistant M tuberculosis strains that have recently shared a common ancestor and undergone clonal expansion. The multidrug-resistant phenotype in these organisms arose by sequential acquisition of resistance-conferring mutations in several genes, most likely as a consequence of antibiotic selection of randomly occurring mutants in concert with inadequately treated infections. Dissemination of these difficult-to-treat bacteria throughout New York City and to at least four additional US cities has adverse implications for tuberculosis control in the 21st century.

AB - Objective. - To determine whether isolates of Mycobacterium tuberculosis from New York and elsewhere that are resistant to four or more primary antimicrobial agents and responsible for widespread disease in the 1990s represent a newly emerged clone or a heterogeneous array of unrelated organisms. Setting. - New York City area and selected locations in the United States. Patients. - M tuberculosis isolates from 1953 patients in New York and multidrug-resistant isolates from six patients from other US communities. Design. - Convenience sample of all M tuberculosis strains (M tuberculosis isolates resistant to rifampin, streptomycin, isoniazid, and ethambutol, and sometimes ethionamide, kanamycin, capreomycin, or ciprofloxacin) submitted to the Public Health Research Institute Tuberculosis Center since 1991 and samples submitted to the Centers for Disease Control and Prevention from throughout the United States. The samples submitted were representative of the New York City strains of M tuberculosis. Main Outcome Measure. - Characterization of resistant M tuberculosis strains studied by IS6110 and polymorphic GC-rich repetitive sequence (PGRS) hybridization patterns, multiplex polymerase chain reaction (PCR) analysis, and automated DNA sequencing of genes containing mutations associated with resistance to rifampin (rpoB), isoniazid (katG and inhA locus), and streptomycin (strA and rrs). Results. - Multidrug-resistant M tuberculosis isolates were recovered from 253 New York City patients and had the same or closely allied IS6110 and PGRS patterns, multiplex PCR type, and gene mutations associated with resistance to rifampin, isoniazid, and streptomycin. Isolates with these same molecular characteristics were recovered from patients in Florida and Nevada, health care workers in Atlanta, Ga, and Miami, Fla, and an individual who recently moved from New York City to Denver, Colo, and caused disease or skin test conversion in at least 12 people in a nursing home environment. Conclusions. - The results document the molecular origin and spread of progeny of a closely related family of multidrug-resistant M tuberculosis strains that have recently shared a common ancestor and undergone clonal expansion. The multidrug-resistant phenotype in these organisms arose by sequential acquisition of resistance-conferring mutations in several genes, most likely as a consequence of antibiotic selection of randomly occurring mutants in concert with inadequately treated infections. Dissemination of these difficult-to-treat bacteria throughout New York City and to at least four additional US cities has adverse implications for tuberculosis control in the 21st century.

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