Ouabain resistance conferred by expression of the cDNA for a murine Na +,K+-ATPase α subunit

Rachel B. Kent, Janet Rettig Emanuel, Yinon Ben Neriah, Robert Levenson, David E. Housman

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The molecular basis for the marked difference between primate and rodent cells in sensitivity to the cardiac glycoside ouabain has been established by genetic techniques. A complementary DNA encoding the entire α1 subunit of the mouse Na+- and K+-dependent adenosine triphosphatase (ATPase) was inserted into the expression vector pSV2. This engineered DNA molecule confers resistance against 10-4 M ouabain to monkey CV-1 cells. Deletion of sequences encoding the carboxyl terminus of the α1 subunit abolish the activity of the complementary DNA. The ability to assay the biological activity of this ATPase in a transfection protocol permits the application of molecular genetic techniques to the analysis of structure-function relationships for the enzyme that establishes the internal Na+/K+ environment of most animal cells. The full-length α1 subunit complementary DNA will also be useful as a dominant selectable marker for somatic cell genetic studies utilizing ouabain-scnsitive cells.

Original languageEnglish (US)
Pages (from-to)901-903
Number of pages3
JournalScience
Volume237
Issue number4817
DOIs
StatePublished - Jan 1 1987

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Ouabain
Adenosine Triphosphatases
Complementary DNA
Genetic Techniques
Cardiac Glycosides
Sequence Deletion
Biological Assay
Primates
Haplorhini
Transfection
Molecular Biology
Rodentia
DNA
Enzymes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Kent, Rachel B. ; Emanuel, Janet Rettig ; Neriah, Yinon Ben ; Levenson, Robert ; Housman, David E. / Ouabain resistance conferred by expression of the cDNA for a murine Na +,K+-ATPase α subunit. In: Science. 1987 ; Vol. 237, No. 4817. pp. 901-903.
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Ouabain resistance conferred by expression of the cDNA for a murine Na +,K+-ATPase α subunit. / Kent, Rachel B.; Emanuel, Janet Rettig; Neriah, Yinon Ben; Levenson, Robert; Housman, David E.

In: Science, Vol. 237, No. 4817, 01.01.1987, p. 901-903.

Research output: Contribution to journalArticle

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