Overexpression of cyclooxygenase 2 in hamartomatous polyps of Peutz-Jeghers syndrome

Thomas McGarrity, Laurie P. Peiffer, Christopher I. Amos, Marsha L. Frazier, Margaret G. Ward, Mary K. Howett

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

OBJECTIVE: Peutz-Jeghers syndrome (PJS) is an autosomal-dominant hamartomatous polyposis syndrome. Affected individuals are at risk for intestinal and extraintestinal malignancies. Prostaglandins and polyamines are small molecules believed to be important in tumor formation and growth. Cyclooxygenase (COX) and ornithine decarboxylase (ODC) are key enzymes in the prostaglandin and polyamine biosynthetic pathways, respectively. The aim of this study was to measure and compare COX-1 and COX-2 expression in normal and hamartomatous tissue of PJS patients. METHODS: We measured COX-1 and COX-2 protein expression in normal and hamartomatous GI tissues from affected PJS individuals and compared it with that in normal controls. COX-2 RNA in these tissues was also measured and compared by reverse transcription polymerase chain reaction (PCR). In addition, COX-2 expression was detected in tissue slides by immunostaining. ODC activity was measured between normal and hamartomatous tissues of PJS compared with control tissues. RESULTS: COX-1 expression was similar in normal and control GI tissues. In contrast, COX-2 overexpression was noted in hamartomatous polyp tissue from PJS patients compared with normal control and PJS tissue. COX-2 expression by reverse transcription PCR was 10-fold greater in a hamartoma compared with other tissues. COX-2 expression was noted in the epithelial cells of hamartomatous polyps, and also coursing throughout the stromal tissue of the lamina propria, including muscle cells. ODC activity was similar in the tissues studied. CONCLUSIONS: Selective COX-2 overexpression was noted in hamartomatous polyp tissue from PJS individuals. The results of the study provide an avenue for possible effective chemoprevention of polyp formation and growth in PJS.

Original languageEnglish (US)
Pages (from-to)671-678
Number of pages8
JournalAmerican Journal of Gastroenterology
Volume98
Issue number3
DOIs
StatePublished - Mar 1 2003

Fingerprint

Peutz-Jeghers Syndrome
Cyclooxygenase 2
Polyps
Cyclooxygenase 1
Ornithine Decarboxylase
Polyamines
Reverse Transcription
Prostaglandins
Polymerase Chain Reaction
Hamartoma
Biosynthetic Pathways
Chemoprevention
Prostaglandin-Endoperoxide Synthases
Growth
Muscle Cells

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

McGarrity, Thomas ; Peiffer, Laurie P. ; Amos, Christopher I. ; Frazier, Marsha L. ; Ward, Margaret G. ; Howett, Mary K. / Overexpression of cyclooxygenase 2 in hamartomatous polyps of Peutz-Jeghers syndrome. In: American Journal of Gastroenterology. 2003 ; Vol. 98, No. 3. pp. 671-678.
@article{3031cbe023fc4e419ca1f678fcb61eb4,
title = "Overexpression of cyclooxygenase 2 in hamartomatous polyps of Peutz-Jeghers syndrome",
abstract = "OBJECTIVE: Peutz-Jeghers syndrome (PJS) is an autosomal-dominant hamartomatous polyposis syndrome. Affected individuals are at risk for intestinal and extraintestinal malignancies. Prostaglandins and polyamines are small molecules believed to be important in tumor formation and growth. Cyclooxygenase (COX) and ornithine decarboxylase (ODC) are key enzymes in the prostaglandin and polyamine biosynthetic pathways, respectively. The aim of this study was to measure and compare COX-1 and COX-2 expression in normal and hamartomatous tissue of PJS patients. METHODS: We measured COX-1 and COX-2 protein expression in normal and hamartomatous GI tissues from affected PJS individuals and compared it with that in normal controls. COX-2 RNA in these tissues was also measured and compared by reverse transcription polymerase chain reaction (PCR). In addition, COX-2 expression was detected in tissue slides by immunostaining. ODC activity was measured between normal and hamartomatous tissues of PJS compared with control tissues. RESULTS: COX-1 expression was similar in normal and control GI tissues. In contrast, COX-2 overexpression was noted in hamartomatous polyp tissue from PJS patients compared with normal control and PJS tissue. COX-2 expression by reverse transcription PCR was 10-fold greater in a hamartoma compared with other tissues. COX-2 expression was noted in the epithelial cells of hamartomatous polyps, and also coursing throughout the stromal tissue of the lamina propria, including muscle cells. ODC activity was similar in the tissues studied. CONCLUSIONS: Selective COX-2 overexpression was noted in hamartomatous polyp tissue from PJS individuals. The results of the study provide an avenue for possible effective chemoprevention of polyp formation and growth in PJS.",
author = "Thomas McGarrity and Peiffer, {Laurie P.} and Amos, {Christopher I.} and Frazier, {Marsha L.} and Ward, {Margaret G.} and Howett, {Mary K.}",
year = "2003",
month = "3",
day = "1",
doi = "10.1111/j.1572-0241.2003.07328.x",
language = "English (US)",
volume = "98",
pages = "671--678",
journal = "American Journal of Gastroenterology",
issn = "0002-9270",
publisher = "Nature Publishing Group",
number = "3",

}

Overexpression of cyclooxygenase 2 in hamartomatous polyps of Peutz-Jeghers syndrome. / McGarrity, Thomas; Peiffer, Laurie P.; Amos, Christopher I.; Frazier, Marsha L.; Ward, Margaret G.; Howett, Mary K.

In: American Journal of Gastroenterology, Vol. 98, No. 3, 01.03.2003, p. 671-678.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Overexpression of cyclooxygenase 2 in hamartomatous polyps of Peutz-Jeghers syndrome

AU - McGarrity, Thomas

AU - Peiffer, Laurie P.

AU - Amos, Christopher I.

AU - Frazier, Marsha L.

AU - Ward, Margaret G.

AU - Howett, Mary K.

PY - 2003/3/1

Y1 - 2003/3/1

N2 - OBJECTIVE: Peutz-Jeghers syndrome (PJS) is an autosomal-dominant hamartomatous polyposis syndrome. Affected individuals are at risk for intestinal and extraintestinal malignancies. Prostaglandins and polyamines are small molecules believed to be important in tumor formation and growth. Cyclooxygenase (COX) and ornithine decarboxylase (ODC) are key enzymes in the prostaglandin and polyamine biosynthetic pathways, respectively. The aim of this study was to measure and compare COX-1 and COX-2 expression in normal and hamartomatous tissue of PJS patients. METHODS: We measured COX-1 and COX-2 protein expression in normal and hamartomatous GI tissues from affected PJS individuals and compared it with that in normal controls. COX-2 RNA in these tissues was also measured and compared by reverse transcription polymerase chain reaction (PCR). In addition, COX-2 expression was detected in tissue slides by immunostaining. ODC activity was measured between normal and hamartomatous tissues of PJS compared with control tissues. RESULTS: COX-1 expression was similar in normal and control GI tissues. In contrast, COX-2 overexpression was noted in hamartomatous polyp tissue from PJS patients compared with normal control and PJS tissue. COX-2 expression by reverse transcription PCR was 10-fold greater in a hamartoma compared with other tissues. COX-2 expression was noted in the epithelial cells of hamartomatous polyps, and also coursing throughout the stromal tissue of the lamina propria, including muscle cells. ODC activity was similar in the tissues studied. CONCLUSIONS: Selective COX-2 overexpression was noted in hamartomatous polyp tissue from PJS individuals. The results of the study provide an avenue for possible effective chemoprevention of polyp formation and growth in PJS.

AB - OBJECTIVE: Peutz-Jeghers syndrome (PJS) is an autosomal-dominant hamartomatous polyposis syndrome. Affected individuals are at risk for intestinal and extraintestinal malignancies. Prostaglandins and polyamines are small molecules believed to be important in tumor formation and growth. Cyclooxygenase (COX) and ornithine decarboxylase (ODC) are key enzymes in the prostaglandin and polyamine biosynthetic pathways, respectively. The aim of this study was to measure and compare COX-1 and COX-2 expression in normal and hamartomatous tissue of PJS patients. METHODS: We measured COX-1 and COX-2 protein expression in normal and hamartomatous GI tissues from affected PJS individuals and compared it with that in normal controls. COX-2 RNA in these tissues was also measured and compared by reverse transcription polymerase chain reaction (PCR). In addition, COX-2 expression was detected in tissue slides by immunostaining. ODC activity was measured between normal and hamartomatous tissues of PJS compared with control tissues. RESULTS: COX-1 expression was similar in normal and control GI tissues. In contrast, COX-2 overexpression was noted in hamartomatous polyp tissue from PJS patients compared with normal control and PJS tissue. COX-2 expression by reverse transcription PCR was 10-fold greater in a hamartoma compared with other tissues. COX-2 expression was noted in the epithelial cells of hamartomatous polyps, and also coursing throughout the stromal tissue of the lamina propria, including muscle cells. ODC activity was similar in the tissues studied. CONCLUSIONS: Selective COX-2 overexpression was noted in hamartomatous polyp tissue from PJS individuals. The results of the study provide an avenue for possible effective chemoprevention of polyp formation and growth in PJS.

UR - http://www.scopus.com/inward/record.url?scp=0037343907&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037343907&partnerID=8YFLogxK

U2 - 10.1111/j.1572-0241.2003.07328.x

DO - 10.1111/j.1572-0241.2003.07328.x

M3 - Article

C2 - 12650805

AN - SCOPUS:0037343907

VL - 98

SP - 671

EP - 678

JO - American Journal of Gastroenterology

JF - American Journal of Gastroenterology

SN - 0002-9270

IS - 3

ER -