Overexpression of the FRO2 Ferric Chelate Reductase Confers Tolerance to Growth on Low Iron and Uncovers Posttranscriptional Control

Erin L. Connolly, Nathan H. Campbell, Natasha Grotz, Charis L. Prichard, Mary Lou Guerinot

Research output: Contribution to journalArticle

263 Citations (Scopus)

Abstract

The Arabidopsis FRO2 gene encodes the low-iron-inducible ferric chelate reductase responsible for reduction of iron at the root surface. Here, we report that FRO2 and IRT1, the major transporter responsible for high-affinity iron uptake from the soil, are coordinately regulated at both the transcriptional and posttranscriptional levels. FRO2 and IRT1 are induced together following the imposition of iron starvation and are coordinately repressed following iron resupply. Steady-state mRNA levels of FRO2 and IRT1 are also coordinately regulated by zinc and cadmium. Like IRT1, FRO2 mRNA is detected in the epidermal cells of roots, consistent with its proposed role in iron uptake from the soil. FRO2 mRNA is detected at high levels in the roots and shoots of 35S-FRO2 transgenic plants. However, ferric chelate reductase activity is only elevated in the 35S-FRO2 plants under conditions of iron deficiency, indicating that FRO2 is subject to posttranscriptional regulation, as shown previously for IRT1. Finally, the 35S-FRO2 plants grow better on low iron as compared with wild-type plants, supporting the idea that reduction of ferric iron to ferrous iron is the rate-limiting step in iron uptake.

Original languageEnglish (US)
Pages (from-to)1102-1110
Number of pages9
JournalPlant physiology
Volume133
Issue number3
DOIs
StatePublished - Nov 1 2003

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chelates
Iron
iron
Growth
Messenger RNA
ferric citrate iron reductase
Soil
Genetically Modified Plants
Starvation
Cadmium
Arabidopsis
starvation
transporters
Zinc
transgenic plants
cadmium
soil
zinc

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Connolly, Erin L. ; Campbell, Nathan H. ; Grotz, Natasha ; Prichard, Charis L. ; Guerinot, Mary Lou. / Overexpression of the FRO2 Ferric Chelate Reductase Confers Tolerance to Growth on Low Iron and Uncovers Posttranscriptional Control. In: Plant physiology. 2003 ; Vol. 133, No. 3. pp. 1102-1110.
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Overexpression of the FRO2 Ferric Chelate Reductase Confers Tolerance to Growth on Low Iron and Uncovers Posttranscriptional Control. / Connolly, Erin L.; Campbell, Nathan H.; Grotz, Natasha; Prichard, Charis L.; Guerinot, Mary Lou.

In: Plant physiology, Vol. 133, No. 3, 01.11.2003, p. 1102-1110.

Research output: Contribution to journalArticle

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