Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7

Skye A. Geherin, Michael H. Lee, R. Paul Wilson, Gudrun F. Debes

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

γδ T cells continuously survey extralymphoid tissues, providing key effector functions during infection and inflammation. Despite their importance, the function and the molecules that drive migration of skin-recirculating γδ T cells are poorly described. Here we found that γδ T cells traveling in the skin-draining afferent lymph of sheep are effectors that produce IFN-γ or IL-17 and express high levels of the skin- and inflammation-seeking molecule E-selectin ligand. Consistent with a role for chemokine receptor CCR7 in mediating T cell exit from extralymphoid tissues, conventional CD4 and CD8T cells in skin-draining lymph were enriched in their expression of CCR7 compared to their skin-residing counterparts. In contrast, co-isolated γδ T cells in skin or lymph lacked expression of CCR7, indicating that they use alternative receptors for egress. Skin-draining γδ T cells were unresponsive to many cutaneous and inflammatory chemokines, including ligands for CCR2, CCR4, CCR5, CCR8, CCR10, and CXCR3, but showed selective chemotaxis toward the cutaneously expressed CCR6 ligand CCL20. Moreover, IL-17+ γδ T cells were the most CCL20-responsive subset of γδ T cells. The data suggest that γδ T cells survey the skin and sites of inflammation and infection, entering via CCR6 and E-selectin ligand and leaving independent of the CCR7-CCL21 axis.

Original languageEnglish (US)
Pages (from-to)87-97
Number of pages11
JournalVeterinary Immunology and Immunopathology
Volume155
Issue number1-2
DOIs
StatePublished - Sep 1 2013

Fingerprint

Interleukin-17
skin (animal)
Sheep
T-lymphocytes
T-Lymphocytes
sheep
Skin
lymph
Lymph
Ligands
E-Selectin
inflammation
Inflammation
tissues
Chemokine Receptors
chemotaxis
T-Lymphocyte Subsets
Chemotaxis
chemokines
Infection

All Science Journal Classification (ASJC) codes

  • Immunology
  • veterinary(all)

Cite this

@article{d5dd334437654d6d958c84c1f81e77ce,
title = "Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7",
abstract = "γδ T cells continuously survey extralymphoid tissues, providing key effector functions during infection and inflammation. Despite their importance, the function and the molecules that drive migration of skin-recirculating γδ T cells are poorly described. Here we found that γδ T cells traveling in the skin-draining afferent lymph of sheep are effectors that produce IFN-γ or IL-17 and express high levels of the skin- and inflammation-seeking molecule E-selectin ligand. Consistent with a role for chemokine receptor CCR7 in mediating T cell exit from extralymphoid tissues, conventional CD4 and CD8T cells in skin-draining lymph were enriched in their expression of CCR7 compared to their skin-residing counterparts. In contrast, co-isolated γδ T cells in skin or lymph lacked expression of CCR7, indicating that they use alternative receptors for egress. Skin-draining γδ T cells were unresponsive to many cutaneous and inflammatory chemokines, including ligands for CCR2, CCR4, CCR5, CCR8, CCR10, and CXCR3, but showed selective chemotaxis toward the cutaneously expressed CCR6 ligand CCL20. Moreover, IL-17+ γδ T cells were the most CCL20-responsive subset of γδ T cells. The data suggest that γδ T cells survey the skin and sites of inflammation and infection, entering via CCR6 and E-selectin ligand and leaving independent of the CCR7-CCL21 axis.",
author = "Geherin, {Skye A.} and Lee, {Michael H.} and Wilson, {R. Paul} and Debes, {Gudrun F.}",
year = "2013",
month = "9",
day = "1",
doi = "10.1016/j.vetimm.2013.06.008",
language = "English (US)",
volume = "155",
pages = "87--97",
journal = "Veterinary Immunology and Immunopathology",
issn = "0165-2427",
publisher = "Elsevier",
number = "1-2",

}

Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7. / Geherin, Skye A.; Lee, Michael H.; Wilson, R. Paul; Debes, Gudrun F.

In: Veterinary Immunology and Immunopathology, Vol. 155, No. 1-2, 01.09.2013, p. 87-97.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7

AU - Geherin, Skye A.

AU - Lee, Michael H.

AU - Wilson, R. Paul

AU - Debes, Gudrun F.

PY - 2013/9/1

Y1 - 2013/9/1

N2 - γδ T cells continuously survey extralymphoid tissues, providing key effector functions during infection and inflammation. Despite their importance, the function and the molecules that drive migration of skin-recirculating γδ T cells are poorly described. Here we found that γδ T cells traveling in the skin-draining afferent lymph of sheep are effectors that produce IFN-γ or IL-17 and express high levels of the skin- and inflammation-seeking molecule E-selectin ligand. Consistent with a role for chemokine receptor CCR7 in mediating T cell exit from extralymphoid tissues, conventional CD4 and CD8T cells in skin-draining lymph were enriched in their expression of CCR7 compared to their skin-residing counterparts. In contrast, co-isolated γδ T cells in skin or lymph lacked expression of CCR7, indicating that they use alternative receptors for egress. Skin-draining γδ T cells were unresponsive to many cutaneous and inflammatory chemokines, including ligands for CCR2, CCR4, CCR5, CCR8, CCR10, and CXCR3, but showed selective chemotaxis toward the cutaneously expressed CCR6 ligand CCL20. Moreover, IL-17+ γδ T cells were the most CCL20-responsive subset of γδ T cells. The data suggest that γδ T cells survey the skin and sites of inflammation and infection, entering via CCR6 and E-selectin ligand and leaving independent of the CCR7-CCL21 axis.

AB - γδ T cells continuously survey extralymphoid tissues, providing key effector functions during infection and inflammation. Despite their importance, the function and the molecules that drive migration of skin-recirculating γδ T cells are poorly described. Here we found that γδ T cells traveling in the skin-draining afferent lymph of sheep are effectors that produce IFN-γ or IL-17 and express high levels of the skin- and inflammation-seeking molecule E-selectin ligand. Consistent with a role for chemokine receptor CCR7 in mediating T cell exit from extralymphoid tissues, conventional CD4 and CD8T cells in skin-draining lymph were enriched in their expression of CCR7 compared to their skin-residing counterparts. In contrast, co-isolated γδ T cells in skin or lymph lacked expression of CCR7, indicating that they use alternative receptors for egress. Skin-draining γδ T cells were unresponsive to many cutaneous and inflammatory chemokines, including ligands for CCR2, CCR4, CCR5, CCR8, CCR10, and CXCR3, but showed selective chemotaxis toward the cutaneously expressed CCR6 ligand CCL20. Moreover, IL-17+ γδ T cells were the most CCL20-responsive subset of γδ T cells. The data suggest that γδ T cells survey the skin and sites of inflammation and infection, entering via CCR6 and E-selectin ligand and leaving independent of the CCR7-CCL21 axis.

UR - http://www.scopus.com/inward/record.url?scp=84881022050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881022050&partnerID=8YFLogxK

U2 - 10.1016/j.vetimm.2013.06.008

DO - 10.1016/j.vetimm.2013.06.008

M3 - Article

C2 - 23838472

AN - SCOPUS:84881022050

VL - 155

SP - 87

EP - 97

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

IS - 1-2

ER -