Oxidation of a Tetrameric Nonphenolic Lignin Model Compound by Lignin Peroxidase

Tünde Mester, Katia Ambert-Balay, Simone Ciofi-Baffoni, Lucia Banci, A. Daniel Jones, Ming Tien

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The present study maps the active site of lignin peroxidase in respect to substrate size using either fungal or recombinant wild type, as well as mutated, recombinant lignin peroxidases. A nonphenolic tetrameric lignin model was synthesized that contains β-O-4 linkages. The fungal and recombinant wild type lignin peroxidase both oxidized the tetrameric model forming four products. The four products were identified by mass spectral analyses and compared with synthetic standards. They were identified as tetrameric, trimeric, dimeric, and monomeric carbonyl compounds. All four of these products were also formed from single turnover experiments. This indicates that lignin peroxidase is able to attack any of the Cα-Cβ linkages in the tetrameric compound and that the substrate-binding site is well exposed. Mutation of the recombinant lignin peroxidase (isozyme H8) in the heme access channel, which is relatively restricted and was previously proposed to be the veratryl alcohol-binding site (E146S), had little effect on the oxidation of the tetramer. In contrast, mutation of a Trp residue (W171S) in the alternate proposed substrate-binding site completely inhibited the oxidation of the tetrameric model. These results are consistent with lignin peroxidase having an exposed active site capable of directly interacting with the lignin polymer without the advent of low molecular weight mediators.

Original languageEnglish (US)
Pages (from-to)22985-22990
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number25
DOIs
StatePublished - Jun 22 2001

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Lignin
Oxidation
Binding Sites
Catalytic Domain
Substrates
Carbonyl compounds
Mutation
Heme
Isoenzymes
lignin peroxidase
Polymers
Molecular Weight
Molecular weight
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Mester, Tünde ; Ambert-Balay, Katia ; Ciofi-Baffoni, Simone ; Banci, Lucia ; Jones, A. Daniel ; Tien, Ming. / Oxidation of a Tetrameric Nonphenolic Lignin Model Compound by Lignin Peroxidase. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 25. pp. 22985-22990.
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Mester, T, Ambert-Balay, K, Ciofi-Baffoni, S, Banci, L, Jones, AD & Tien, M 2001, 'Oxidation of a Tetrameric Nonphenolic Lignin Model Compound by Lignin Peroxidase', Journal of Biological Chemistry, vol. 276, no. 25, pp. 22985-22990. https://doi.org/10.1074/jbc.M010739200

Oxidation of a Tetrameric Nonphenolic Lignin Model Compound by Lignin Peroxidase. / Mester, Tünde; Ambert-Balay, Katia; Ciofi-Baffoni, Simone; Banci, Lucia; Jones, A. Daniel; Tien, Ming.

In: Journal of Biological Chemistry, Vol. 276, No. 25, 22.06.2001, p. 22985-22990.

Research output: Contribution to journalArticle

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AU - Ambert-Balay, Katia

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