Ozone-induced ethylene and foliar injury responses are altered in 1-aminocyclopropane-1-carboxylate synthase antisense potato plants

Judith Pawloski Sinn, Carl D. Schlagnhaufer, Richard N. Arteca, Eva J. Pell

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

In potato (Solanum tuberosum), two 1-aminocyclopropane-1-carboxylate synthase (ACS) genes are induced by ozone (O3). Antisense inhibition of these ethylene (C2H4) biosynthetic genes allowed us to examine the relationship between O3-induced C2H 4 emission and foliar injury. • Thirty-two lines (antisense for ST-ACS4 or ST-ACS5) were screened in the glasshouse for acute O 3-induced C2H4 and lesions. Stomatal conductance and ACS transcripts were quantified for selected C2H 4-altered lines. Six lines were field-tested for chronic O 3 effects. • Ten lines produced less, and four lines produced more, acute-O3-induced C2H4 than nontransformed (NT) plants. Ethylene levels did not appear to be correlated with stomatal conductance. ST-ACS4 and -5 transcript were reduced in transgenic plants, except in two C2H4 over-producing lines. In the field, these C2H4 over-producing lines displayed stunting and leaf rolling in charcoal-filtered (CF) air and chronic O3, and they sustained the most severe O3 injury. • When C2H 4 production was strongly suppressed or enhanced, corresponding reductions or increases in lesion severity were observed, suggesting a critical role for C2H4 in the lesion formation process during O3 stress.

Original languageEnglish (US)
Pages (from-to)267-277
Number of pages11
JournalNew Phytologist
Volume164
Issue number2
DOIs
StatePublished - Nov 1 2004

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

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