P-glycoprotein expression in cartilaginous tumors

Randyn Rosier, Regis J. O'Keefe, Lisa A. Teot, Edward Fox, Theresa A. Nester, J. Edward Puzas, Paul R. Reynolds, David G. Hicks

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background and Objectives: Malignant cartilage tumors demonstrate chemotherapeutic resistance through undetermined mechanisms. Pglycoprotein is the protein product of the multiple drug resistance gene I (MDR-I) and confers multidrug chemotherapeutic resistance in a variety of malignancies. Methods: MDR-I expression was examined in 55 benign and malignant cartilage tumor specimens by immunohistochemistry using C219, C494, and JSB-1 antibodies, and by in situ hybridization with an MDR-I specific oligonucleotide cDNA probe. Results: Constitutive expression of P- glycoprotein was observed in all benign and malignant cartilage tumor specimens with a similar pattern of immunohistochemical staining present with all three antibodies. In benign tumors and low grade chondrosarcomas, the staining pattern was weak to intermediate and localized to clusters of cells. However, higher grade tumors (Grade II and III) expressed P-glycoprotein in a higher percentage of cells and with more intense staining. P-glycoprotein expression was absent in normal human articular cartilage, but was focally present in costal and growth plate cartilage. The immunohistochemistry results were confirmed by in situ hybridization in 10 cases. Conclusions: P- glycoprotein is expressed constitutively in cartilaginous tumors, with greatest expression in high grade malignancies. The findings may account for the resistance of cartilage tumors to chemotherapeutic agents.

Original languageEnglish (US)
Pages (from-to)95-105
Number of pages11
JournalJournal of Surgical Oncology
Volume65
Issue number2
DOIs
StatePublished - Jun 1 1997

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P-Glycoprotein
Cartilage
Multiple Drug Resistance
Neoplasms
Staining and Labeling
In Situ Hybridization
Immunohistochemistry
Chondrosarcoma
Growth Plate
Oligonucleotide Probes
Antibodies
Articular Cartilage
Genes
Complementary DNA
Gene Expression

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

Cite this

Rosier, R., O'Keefe, R. J., Teot, L. A., Fox, E., Nester, T. A., Puzas, J. E., ... Hicks, D. G. (1997). P-glycoprotein expression in cartilaginous tumors. Journal of Surgical Oncology, 65(2), 95-105. https://doi.org/10.1002/(SICI)1096-9098(199706)65:2<95::AID-JSO5>3.0.CO;2-I
Rosier, Randyn ; O'Keefe, Regis J. ; Teot, Lisa A. ; Fox, Edward ; Nester, Theresa A. ; Puzas, J. Edward ; Reynolds, Paul R. ; Hicks, David G. / P-glycoprotein expression in cartilaginous tumors. In: Journal of Surgical Oncology. 1997 ; Vol. 65, No. 2. pp. 95-105.
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abstract = "Background and Objectives: Malignant cartilage tumors demonstrate chemotherapeutic resistance through undetermined mechanisms. Pglycoprotein is the protein product of the multiple drug resistance gene I (MDR-I) and confers multidrug chemotherapeutic resistance in a variety of malignancies. Methods: MDR-I expression was examined in 55 benign and malignant cartilage tumor specimens by immunohistochemistry using C219, C494, and JSB-1 antibodies, and by in situ hybridization with an MDR-I specific oligonucleotide cDNA probe. Results: Constitutive expression of P- glycoprotein was observed in all benign and malignant cartilage tumor specimens with a similar pattern of immunohistochemical staining present with all three antibodies. In benign tumors and low grade chondrosarcomas, the staining pattern was weak to intermediate and localized to clusters of cells. However, higher grade tumors (Grade II and III) expressed P-glycoprotein in a higher percentage of cells and with more intense staining. P-glycoprotein expression was absent in normal human articular cartilage, but was focally present in costal and growth plate cartilage. The immunohistochemistry results were confirmed by in situ hybridization in 10 cases. Conclusions: P- glycoprotein is expressed constitutively in cartilaginous tumors, with greatest expression in high grade malignancies. The findings may account for the resistance of cartilage tumors to chemotherapeutic agents.",
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Rosier, R, O'Keefe, RJ, Teot, LA, Fox, E, Nester, TA, Puzas, JE, Reynolds, PR & Hicks, DG 1997, 'P-glycoprotein expression in cartilaginous tumors', Journal of Surgical Oncology, vol. 65, no. 2, pp. 95-105. https://doi.org/10.1002/(SICI)1096-9098(199706)65:2<95::AID-JSO5>3.0.CO;2-I

P-glycoprotein expression in cartilaginous tumors. / Rosier, Randyn; O'Keefe, Regis J.; Teot, Lisa A.; Fox, Edward; Nester, Theresa A.; Puzas, J. Edward; Reynolds, Paul R.; Hicks, David G.

In: Journal of Surgical Oncology, Vol. 65, No. 2, 01.06.1997, p. 95-105.

Research output: Contribution to journalArticle

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T1 - P-glycoprotein expression in cartilaginous tumors

AU - Rosier, Randyn

AU - O'Keefe, Regis J.

AU - Teot, Lisa A.

AU - Fox, Edward

AU - Nester, Theresa A.

AU - Puzas, J. Edward

AU - Reynolds, Paul R.

AU - Hicks, David G.

PY - 1997/6/1

Y1 - 1997/6/1

N2 - Background and Objectives: Malignant cartilage tumors demonstrate chemotherapeutic resistance through undetermined mechanisms. Pglycoprotein is the protein product of the multiple drug resistance gene I (MDR-I) and confers multidrug chemotherapeutic resistance in a variety of malignancies. Methods: MDR-I expression was examined in 55 benign and malignant cartilage tumor specimens by immunohistochemistry using C219, C494, and JSB-1 antibodies, and by in situ hybridization with an MDR-I specific oligonucleotide cDNA probe. Results: Constitutive expression of P- glycoprotein was observed in all benign and malignant cartilage tumor specimens with a similar pattern of immunohistochemical staining present with all three antibodies. In benign tumors and low grade chondrosarcomas, the staining pattern was weak to intermediate and localized to clusters of cells. However, higher grade tumors (Grade II and III) expressed P-glycoprotein in a higher percentage of cells and with more intense staining. P-glycoprotein expression was absent in normal human articular cartilage, but was focally present in costal and growth plate cartilage. The immunohistochemistry results were confirmed by in situ hybridization in 10 cases. Conclusions: P- glycoprotein is expressed constitutively in cartilaginous tumors, with greatest expression in high grade malignancies. The findings may account for the resistance of cartilage tumors to chemotherapeutic agents.

AB - Background and Objectives: Malignant cartilage tumors demonstrate chemotherapeutic resistance through undetermined mechanisms. Pglycoprotein is the protein product of the multiple drug resistance gene I (MDR-I) and confers multidrug chemotherapeutic resistance in a variety of malignancies. Methods: MDR-I expression was examined in 55 benign and malignant cartilage tumor specimens by immunohistochemistry using C219, C494, and JSB-1 antibodies, and by in situ hybridization with an MDR-I specific oligonucleotide cDNA probe. Results: Constitutive expression of P- glycoprotein was observed in all benign and malignant cartilage tumor specimens with a similar pattern of immunohistochemical staining present with all three antibodies. In benign tumors and low grade chondrosarcomas, the staining pattern was weak to intermediate and localized to clusters of cells. However, higher grade tumors (Grade II and III) expressed P-glycoprotein in a higher percentage of cells and with more intense staining. P-glycoprotein expression was absent in normal human articular cartilage, but was focally present in costal and growth plate cartilage. The immunohistochemistry results were confirmed by in situ hybridization in 10 cases. Conclusions: P- glycoprotein is expressed constitutively in cartilaginous tumors, with greatest expression in high grade malignancies. The findings may account for the resistance of cartilage tumors to chemotherapeutic agents.

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