Pantropic retroviral vectors mediate gene transfer and expression in Entamoeba histolytica

Xuchu Que, Doojin Kim, Alejandro Alagon, Ken Hirata, Hiroko Shike, Chisato Shimizu, Antonio Gonzalez, Jane C. Burns, Sharon L. Reed

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Transformation of Entamoeba histolytica has been previously reported, but the foreign genes have all been replicated episomally. Pantropic retroviral vectors based on the Moloney murine leukemia virus with the envelope glycoprotein of vesicular stomatitis virus (VSV-G) have an extremely broad host range and can be concentrated to high titer. To investigate whether these pseudotyped, pantropic vectors can mediate gene transfer and expression in E. histolytica, we constructed a retroviral vector, in which a hygromycin phosphotransferase is expressed from the E. histolytica actin promoter. Data confirm the infection, integration, and expression of a foreign gene mediated by the provirus. To our knowledge, this is the most evolutionarily distant example of successful integration and expression of a mammalian retrovirus. Pantropic retroviral vectors may thus facilitate genetic analysis in species lacking transformation systems. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)237-245
Number of pages9
JournalMolecular and biochemical parasitology
Volume99
Issue number2
DOIs
StatePublished - Apr 30 1999

Fingerprint

Entamoeba histolytica
hygromycin-B kinase
Gene Expression
Moloney murine leukemia virus
Proviruses
Host Specificity
Retroviridae
Genes
Actins
Infection

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Molecular Biology

Cite this

Que, Xuchu ; Kim, Doojin ; Alagon, Alejandro ; Hirata, Ken ; Shike, Hiroko ; Shimizu, Chisato ; Gonzalez, Antonio ; Burns, Jane C. ; Reed, Sharon L. / Pantropic retroviral vectors mediate gene transfer and expression in Entamoeba histolytica. In: Molecular and biochemical parasitology. 1999 ; Vol. 99, No. 2. pp. 237-245.
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Que, X, Kim, D, Alagon, A, Hirata, K, Shike, H, Shimizu, C, Gonzalez, A, Burns, JC & Reed, SL 1999, 'Pantropic retroviral vectors mediate gene transfer and expression in Entamoeba histolytica', Molecular and biochemical parasitology, vol. 99, no. 2, pp. 237-245. https://doi.org/10.1016/S0166-6851(99)00021-3

Pantropic retroviral vectors mediate gene transfer and expression in Entamoeba histolytica. / Que, Xuchu; Kim, Doojin; Alagon, Alejandro; Hirata, Ken; Shike, Hiroko; Shimizu, Chisato; Gonzalez, Antonio; Burns, Jane C.; Reed, Sharon L.

In: Molecular and biochemical parasitology, Vol. 99, No. 2, 30.04.1999, p. 237-245.

Research output: Contribution to journalArticle

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AU - Kim, Doojin

AU - Alagon, Alejandro

AU - Hirata, Ken

AU - Shike, Hiroko

AU - Shimizu, Chisato

AU - Gonzalez, Antonio

AU - Burns, Jane C.

AU - Reed, Sharon L.

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