Pantropic retroviral vectors mediate somatic cell transformation and expression of foreign genes in dipteran insects

T. V. Jordan, H. Shike, V. Boulo, V. Cedeno, Q. Fang, B. S. Davis, M. Jacobs-Lorena, S. Higgs, K. J. Fryxell, J. C. Burns

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The control of insects that transmit disease and damage crops has become Increasingly difficult. The ability to genetically engineer Insects would facilitate strategies to protect crops and block arthropod vectorborne disease transmission. Transformation vectors based on insect transposable elements have been developed, but most have limited host ranges. A promising alternative is the pantropic retroviral vector, which is packaged with the envelope glycoprotein from vesicular stomatitis virus and is replication-defective. We show here that pantropic murine retroviral vectors can mediate high-level expression of foreign genes in somatically transformed Insect larvae and adults of three dipteran genera. This success demonstrates the potential for germline transformation mediated by pantropic retrovital vectors.

Original languageEnglish (US)
Pages (from-to)215-222
Number of pages8
JournalInsect Molecular Biology
Volume7
Issue number3
DOIs
StatePublished - Aug 1998

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Insect Science

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