Papillomavirus DNA complementation in vivo

Jiafen Hu, Nancy M. Cladel, Lynn Budgeon, Karla K. Balogh, Neil D. Christensen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Recent phylogenic studies indicate that DNA recombination could have occurred in ancient papillomavirus types. However, no experimental data are available to demonstrate this event because of the lack of human papillomavirus infection models. We have used the cottontail rabbit papillomavirus (CRPV)/rabbit model to study pathogenesis and immunogenicity of different mutant genomes in vivo. Although the domestic rabbit is not a natural host for CRPV infection, it is possible to initiate infection with naked CRPV DNA cloned into a plasmid and monitor papilloma outgrowth on these animals. Taking advantage of a large panel of mutants based on a CRPV strain (Hershey CRPV), we tested the hypothesis that two non-viable mutant genomes could induce papillomas by either recombination or complementation. We found that co-infection with a dysfunctional mutant with an E2 transactivation domain mutation and another mutant with an E7 ATG knock out generated papillomas in rabbits. DNA extracted from these papillomas contained genotypes from both parental genomes. Three additional pairs of dysfunctional mutants also showed similar results. Individual wild type genes were also shown to rescue the function of corresponding dysfunctional mutants. Therefore, we suggest that complementation occurred between these two non-viable mutant PV genomes in vivo.

Original languageEnglish (US)
Pages (from-to)117-122
Number of pages6
JournalVirus Research
Volume144
Issue number1-2
DOIs
StatePublished - Sep 1 2009

Fingerprint

Cottontail rabbit papillomavirus
Papilloma
Genome
DNA
Papillomavirus Infections
Rabbits
Genetic Recombination
Coinfection
Transcriptional Activation
Plasmids
Genotype
Mutation
Infection
Genes

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Virology
  • Infectious Diseases

Cite this

Hu, Jiafen ; Cladel, Nancy M. ; Budgeon, Lynn ; Balogh, Karla K. ; Christensen, Neil D. / Papillomavirus DNA complementation in vivo. In: Virus Research. 2009 ; Vol. 144, No. 1-2. pp. 117-122.
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Papillomavirus DNA complementation in vivo. / Hu, Jiafen; Cladel, Nancy M.; Budgeon, Lynn; Balogh, Karla K.; Christensen, Neil D.

In: Virus Research, Vol. 144, No. 1-2, 01.09.2009, p. 117-122.

Research output: Contribution to journalArticle

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