Papillomavirus particles assembled in 293TT cells are infectious in vivo

Timothy D. Culp, Nancy M. Cladel, Karla K. Balogh, Lynn R. Budgeon, Andres F. Mejia, Neil D. Christensen

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Papillomaviruses (PVs) demonstrate both tissue and species tropisms. Because PVs replicate only in terminally differentiating epithelium, the recent production of infectious PV particles in 293 cells marks an important breakthrough. In this article, we demonstrate that infectious PV particles produced in 293TT cells can cause papillomatous growths in the natural host animal. Moreover, we show that species-matched PV genomes can be successfully delivered in vivo by a heterologous, species-mismatched PV capsid. Additionally, our results indicate that the addition of the simian virus 40 origin of replication to the papillomavirus genome increases the production of infectious papillomavirus particles by increasing genome amplification in the transfected 293TT cells.

Original languageEnglish (US)
Pages (from-to)11381-11384
Number of pages4
JournalJournal of virology
Volume80
Issue number22
DOIs
StatePublished - Nov 2006

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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