Papillomavirus particles assembled in 293TT cells are infectious in vivo

Timothy D. Culp, Nancy M. Cladel, Karla K. Balogh, Lynn R. Budgeon, Andres F. Mejia, Neil Christensen

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Papillomaviruses (PVs) demonstrate both tissue and species tropisms. Because PVs replicate only in terminally differentiating epithelium, the recent production of infectious PV particles in 293 cells marks an important breakthrough. In this article, we demonstrate that infectious PV particles produced in 293TT cells can cause papillomatous growths in the natural host animal. Moreover, we show that species-matched PV genomes can be successfully delivered in vivo by a heterologous, species-mismatched PV capsid. Additionally, our results indicate that the addition of the simian virus 40 origin of replication to the papillomavirus genome increases the production of infectious papillomavirus particles by increasing genome amplification in the transfected 293TT cells.

Original languageEnglish (US)
Pages (from-to)11381-11384
Number of pages4
JournalJournal of virology
Volume80
Issue number22
DOIs
StatePublished - Nov 1 2006

Fingerprint

Papillomaviridae
Genome
Replication Origin
Tropism
Simian virus 40
Capsid
cells
Epithelium
genome
Growth
replication origin
tropisms
capsid
epithelium

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Culp, T. D., Cladel, N. M., Balogh, K. K., Budgeon, L. R., Mejia, A. F., & Christensen, N. (2006). Papillomavirus particles assembled in 293TT cells are infectious in vivo. Journal of virology, 80(22), 11381-11384. https://doi.org/10.1128/JVI.01328-06
Culp, Timothy D. ; Cladel, Nancy M. ; Balogh, Karla K. ; Budgeon, Lynn R. ; Mejia, Andres F. ; Christensen, Neil. / Papillomavirus particles assembled in 293TT cells are infectious in vivo. In: Journal of virology. 2006 ; Vol. 80, No. 22. pp. 11381-11384.
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Culp, TD, Cladel, NM, Balogh, KK, Budgeon, LR, Mejia, AF & Christensen, N 2006, 'Papillomavirus particles assembled in 293TT cells are infectious in vivo', Journal of virology, vol. 80, no. 22, pp. 11381-11384. https://doi.org/10.1128/JVI.01328-06

Papillomavirus particles assembled in 293TT cells are infectious in vivo. / Culp, Timothy D.; Cladel, Nancy M.; Balogh, Karla K.; Budgeon, Lynn R.; Mejia, Andres F.; Christensen, Neil.

In: Journal of virology, Vol. 80, No. 22, 01.11.2006, p. 11381-11384.

Research output: Contribution to journalArticle

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