Parainfluenza virus 5 M protein interaction with host protein 14-3-3 negatively affects virus particle formation

Zifei Pei, Megan S. Harrison, Anthony Paul Schmitt

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Paramyxovirus matrix (M) proteins organize virus assembly, linking viral glycoproteins and viral ribonucleoproteins together at virus assembly sites on cellular membranes. Using a yeast two-hybrid screening approach, we identified 14-3-3 as a binding partner for the M protein of parainfluenza virus 5 (PIV5). Binding in both transfected and PIV5-infected cells was confirmed by coimmunoprecipitation and was mapped to a C-terminal region within the M protein, namely, 366-KTKSLP-371. This sequence resembles known 14-3-3 binding sites, in which the key residue for binding is a phosphorylated serine residue. Mutation of S369 within the PIV5 M protein disrupted 14-3-3 binding and improved the budding of both virus-like particles (VLPs) and recombinant viruses, suggesting that 14-3-3 binding impairs virus budding. 14-3-3 protein overexpression reduced the budding of VLPs. Using 33P labeling, phosphorylated M protein was detected in PIV5-infected cells, and this phosphorylation was nearly absent in cells infected with a recombinant virus harboring an S369A mutation within the M protein. Assembly of the M protein into clusters and filaments at infected cell surfaces was enhanced in cells infected with a recombinant virus defective in 14-3-3 binding. These findings support a model in which a portion of M protein within PIV5-infected cells is phosphorylated at residue S369, binds the 14-3-3 protein, and is held away from sites of virus budding.

Original languageEnglish (US)
Pages (from-to)2050-2059
Number of pages10
JournalJournal of virology
Volume85
Issue number5
DOIs
StatePublished - Mar 1 2011

Fingerprint

Parainfluenza virus 5
Parainfluenza Virus 5
14-3-3 Proteins
virion
Virion
Proteins
proteins
Virus Release
Virus Assembly
viruses
virus-like particles
cells
Viruses
Defective Viruses
Mutation
Ribonucleoproteins
Serine
Respirovirus
mutation
ribonucleoproteins

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

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title = "Parainfluenza virus 5 M protein interaction with host protein 14-3-3 negatively affects virus particle formation",
abstract = "Paramyxovirus matrix (M) proteins organize virus assembly, linking viral glycoproteins and viral ribonucleoproteins together at virus assembly sites on cellular membranes. Using a yeast two-hybrid screening approach, we identified 14-3-3 as a binding partner for the M protein of parainfluenza virus 5 (PIV5). Binding in both transfected and PIV5-infected cells was confirmed by coimmunoprecipitation and was mapped to a C-terminal region within the M protein, namely, 366-KTKSLP-371. This sequence resembles known 14-3-3 binding sites, in which the key residue for binding is a phosphorylated serine residue. Mutation of S369 within the PIV5 M protein disrupted 14-3-3 binding and improved the budding of both virus-like particles (VLPs) and recombinant viruses, suggesting that 14-3-3 binding impairs virus budding. 14-3-3 protein overexpression reduced the budding of VLPs. Using 33P labeling, phosphorylated M protein was detected in PIV5-infected cells, and this phosphorylation was nearly absent in cells infected with a recombinant virus harboring an S369A mutation within the M protein. Assembly of the M protein into clusters and filaments at infected cell surfaces was enhanced in cells infected with a recombinant virus defective in 14-3-3 binding. These findings support a model in which a portion of M protein within PIV5-infected cells is phosphorylated at residue S369, binds the 14-3-3 protein, and is held away from sites of virus budding.",
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Parainfluenza virus 5 M protein interaction with host protein 14-3-3 negatively affects virus particle formation. / Pei, Zifei; Harrison, Megan S.; Schmitt, Anthony Paul.

In: Journal of virology, Vol. 85, No. 5, 01.03.2011, p. 2050-2059.

Research output: Contribution to journalArticle

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