Paralemmin interacts with D3 dopamine receptors

Implications for membrane localization and cAMP signaling

Maria Basile, Ridwan Lin, Nadine Kabbani, Kelly Karpa, Manfred Kilimann, Ian Simpson, Mark Kester

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Paralemmin is a novel lipid-anchored protein, which is highly expressed in neuronal plasma membranes. In this study, we demonstrate that paralemmin specifically interacts with the third intracellular loop of the D3 dopamine receptor. Utilizing co-immunoprecipitation and glutathione-S-transferase (GST) pulldown strategies, we demonstrate that paralemmin interacts exclusively with D3, but not D2 or D4 dopamine receptors or β-adrenergic receptors. Immunocytochemistry demonstrated co-localization of paralemmin and D3 receptor in vivo in hippocampus and cerebellum and in vitro in glial and neuronal cultures. Deletion mutational analysis indicates that amino acids 154-230 of paralemmin strongly interacted with amino acids 211-227 and 281-330 of the third intracellular loop of D3 receptor. The consequences of these interactions were investigated by co-expression in HEK293 cells. Cell surface biotinylation experiments demonstrate that paralemmin decreased D3 receptor concentration at the plasma membrane. Consistent with this observation, paralemmin expression decreased dopamine-stimulated adenylate cyclase activity. However, paralemmin also decreased basal, isoproterenol and forskolin-stimulated adenylate cyclase activity, suggesting a more general cellular function for paralemmin. Taken together, paralemmin has been implicated as a potent modulator of cellular cAMP signaling within the brain.

Original languageEnglish (US)
Pages (from-to)60-68
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume446
Issue number1
DOIs
StatePublished - Feb 1 2006

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Dopamine D3 Receptors
Cell membranes
Adenylyl Cyclases
Lipid-Linked Proteins
Dopamine D4 Receptors
Cell Membrane
Cell signaling
Biotinylation
Membranes
Amino Acids
Dopamine D2 Receptors
HEK293 Cells
Colforsin
Glutathione Transferase
Isoproterenol
Immunoprecipitation
Neuroglia
Cerebellum
Adrenergic Receptors
Modulators

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Basile, Maria ; Lin, Ridwan ; Kabbani, Nadine ; Karpa, Kelly ; Kilimann, Manfred ; Simpson, Ian ; Kester, Mark. / Paralemmin interacts with D3 dopamine receptors : Implications for membrane localization and cAMP signaling. In: Archives of Biochemistry and Biophysics. 2006 ; Vol. 446, No. 1. pp. 60-68.
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Paralemmin interacts with D3 dopamine receptors : Implications for membrane localization and cAMP signaling. / Basile, Maria; Lin, Ridwan; Kabbani, Nadine; Karpa, Kelly; Kilimann, Manfred; Simpson, Ian; Kester, Mark.

In: Archives of Biochemistry and Biophysics, Vol. 446, No. 1, 01.02.2006, p. 60-68.

Research output: Contribution to journalArticle

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