Parallel analysis with optically gated sample introduction on a multichannel microchip

Hongwei Xu, Thomas P. Roddy, Julie A. Lapos, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

As an alternative to the T-type injection on microchips, optically gated sample introduction previously has been demonstrated to provide fast, serial, and reproducible injections on a single-channel microchip. Here, the ability to perform high throughput, multichannel analysis with optically gated sample introduction is described using a voice coil actuator. The microchip is fixed on a stage, which moves back and forth via the voice coil actuator, scanning two laser beams across the channels on the microchip. For parallel analysis on a multichannel microchip, both the gating beam and the probe beam are scanned at 10 Hz to perform multiple injections and parallel detection. Simultaneous, fast separations of 4-choloro-7-nitrobenzofurazan (NBD)-labeled amino acids are demonstrated in multiple channels on a microchip. Serial separations of different samples in multiple channels are also reported. Optically gated sample introduction on multiple, parallel channels shows the potential for high-speed, high-throughput separations that are easily automated by using a single electronic shutter.

Original languageEnglish (US)
Pages (from-to)5517-5522
Number of pages6
JournalAnalytical Chemistry
Volume74
Issue number21
DOIs
StatePublished - Nov 1 2002

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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