Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells

Bo Lu, Tong Xu, Siyang Zheng, Amir Goldkorn, Yu Chong Tai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

19 Citations (Scopus)

Abstract

This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane "slot" filter. More than 90% viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase activity measurement of a single cancer cell taken from the filter after capture was proven. The on-filter and off-filter cultures of the captured cancer cells were also demonstrated.

Original languageEnglish (US)
Title of host publicationMEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
Pages935-938
Number of pages4
DOIs
StatePublished - Jun 1 2010
Event23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China
Duration: Jan 24 2010Jan 28 2010

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
CountryChina
CityHong Kong
Period1/24/101/28/10

Fingerprint

Cell culture
slots
Tumors
tumors
Cells
membranes
Membranes
filters
Blood
cancer
blood
Telomerase
blood cells
parylene

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Lu, B., Xu, T., Zheng, S., Goldkorn, A., & Tai, Y. C. (2010). Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest (pp. 935-938). [5442361] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442361
Lu, Bo ; Xu, Tong ; Zheng, Siyang ; Goldkorn, Amir ; Tai, Yu Chong. / Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells. MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. pp. 935-938 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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abstract = "This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane {"}slot{"} filter. More than 90{\%} viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase activity measurement of a single cancer cell taken from the filter after capture was proven. The on-filter and off-filter cultures of the captured cancer cells were also demonstrated.",
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Lu, B, Xu, T, Zheng, S, Goldkorn, A & Tai, YC 2010, Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells. in MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest., 5442361, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 935-938, 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010, Hong Kong, China, 1/24/10. https://doi.org/10.1109/MEMSYS.2010.5442361

Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells. / Lu, Bo; Xu, Tong; Zheng, Siyang; Goldkorn, Amir; Tai, Yu Chong.

MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 935-938 5442361 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane "slot" filter. More than 90% viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase activity measurement of a single cancer cell taken from the filter after capture was proven. The on-filter and off-filter cultures of the captured cancer cells were also demonstrated.

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Lu B, Xu T, Zheng S, Goldkorn A, Tai YC. Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 935-938. 5442361. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442361