Abstract
This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane "slot" filter. More than 90% viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase activity measurement of a single cancer cell taken from the filter after capture was proven. The on-filter and off-filter cultures of the captured cancer cells were also demonstrated.
| Original language | English (US) |
|---|---|
| Title of host publication | MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest |
| Pages | 935-938 |
| Number of pages | 4 |
| DOIs | |
| State | Published - Jun 1 2010 |
| Event | 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China Duration: Jan 24 2010 → Jan 28 2010 |
Publication series
| Name | Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) |
|---|---|
| ISSN (Print) | 1084-6999 |
Other
| Other | 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 |
|---|---|
| Country | China |
| City | Hong Kong |
| Period | 1/24/10 → 1/28/10 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Mechanical Engineering
- Electrical and Electronic Engineering
Cite this
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Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells. / Lu, Bo; Xu, Tong; Zheng, Siyang; Goldkorn, Amir; Tai, Yu Chong.
MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 935-938 5442361 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells
AU - Lu, Bo
AU - Xu, Tong
AU - Zheng, Siyang
AU - Goldkorn, Amir
AU - Tai, Yu Chong
PY - 2010/6/1
Y1 - 2010/6/1
N2 - This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane "slot" filter. More than 90% viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase activity measurement of a single cancer cell taken from the filter after capture was proven. The on-filter and off-filter cultures of the captured cancer cells were also demonstrated.
AB - This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane "slot" filter. More than 90% viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase activity measurement of a single cancer cell taken from the filter after capture was proven. The on-filter and off-filter cultures of the captured cancer cells were also demonstrated.
UR - http://www.scopus.com/inward/record.url?scp=77952771018&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77952771018&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2010.5442361
DO - 10.1109/MEMSYS.2010.5442361
M3 - Conference contribution
AN - SCOPUS:77952771018
SN - 9781424457649
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 935
EP - 938
BT - MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
ER -