Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells

Bo Lu; Tong Xu; Siyang Zheng; Amir Goldkorn; Yu Chong Tai

doi:10.1109/MEMSYS.2010.5442361

Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells

Bo Lu, Tong Xu, Siyang Zheng, Amir Goldkorn, Yu Chong Tai

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

20 Scopus citations

Abstract

This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane "slot" filter. More than 90% viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase activity measurement of a single cancer cell taken from the filter after capture was proven. The on-filter and off-filter cultures of the captured cancer cells were also demonstrated.

Original language	English (US)
Title of host publication	MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
Pages	935-938
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2010.5442361
State	Published - 2010
Event	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China Duration: Jan 24 2010 → Jan 28 2010

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
Country	China
City	Hong Kong
Period	1/24/10 → 1/28/10

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2010.5442361

Fingerprint Dive into the research topics of 'Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells'. Together they form a unique fingerprint.

Cite this

Lu, B., Xu, T., Zheng, S., Goldkorn, A., & Tai, Y. C. (2010). Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest (pp. 935-938). [5442361] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442361

Lu, Bo ; Xu, Tong ; Zheng, Siyang ; Goldkorn, Amir ; Tai, Yu Chong. / Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells. MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. pp. 935-938 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

@inproceedings{738373d3f05040aa8279d5e673cf55a0,

title = "Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells",

abstract = "This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane {"}slot{"} filter. More than 90% viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase activity measurement of a single cancer cell taken from the filter after capture was proven. The on-filter and off-filter cultures of the captured cancer cells were also demonstrated.",

author = "Bo Lu and Tong Xu and Siyang Zheng and Amir Goldkorn and Tai, {Yu Chong}",

note = "Copyright: Copyright 2010 Elsevier B.V., All rights reserved.; 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 ; Conference date: 24-01-2010 Through 28-01-2010",

year = "2010",

doi = "10.1109/MEMSYS.2010.5442361",

language = "English (US)",

isbn = "9781424457649",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "935--938",

booktitle = "MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest",

}

Lu, B, Xu, T, Zheng, S, Goldkorn, A & Tai, YC 2010, Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells. in MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest., 5442361, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 935-938, 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010, Hong Kong, China, 1/24/10. https://doi.org/10.1109/MEMSYS.2010.5442361

Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells. / Lu, Bo; Xu, Tong; Zheng, Siyang; Goldkorn, Amir; Tai, Yu Chong.

MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 935-938 5442361 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells

AU - Lu, Bo

AU - Xu, Tong

AU - Zheng, Siyang

AU - Goldkorn, Amir

AU - Tai, Yu Chong

PY - 2010

Y1 - 2010

N2 - This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane "slot" filter. More than 90% viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase activity measurement of a single cancer cell taken from the filter after capture was proven. The on-filter and off-filter cultures of the captured cancer cells were also demonstrated.

AB - This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane "slot" filter. More than 90% viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase activity measurement of a single cancer cell taken from the filter after capture was proven. The on-filter and off-filter cultures of the captured cancer cells were also demonstrated.

UR - http://www.scopus.com/inward/record.url?scp=77952771018&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952771018&partnerID=8YFLogxK

U2 - 10.1109/MEMSYS.2010.5442361

DO - 10.1109/MEMSYS.2010.5442361

M3 - Conference contribution

AN - SCOPUS:77952771018

SN - 9781424457649

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 935

EP - 938

BT - MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest

T2 - 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010

Y2 - 24 January 2010 through 28 January 2010

ER -

Lu B, Xu T, Zheng S, Goldkorn A, Tai YC. Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 935-938. 5442361. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442361