TY - GEN
T1 - Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells
AU - Lu, Bo
AU - Xu, Tong
AU - Zheng, Siyang
AU - Goldkorn, Amir
AU - Tai, Yu Chong
PY - 2010
Y1 - 2010
N2 - This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane "slot" filter. More than 90% viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase activity measurement of a single cancer cell taken from the filter after capture was proven. The on-filter and off-filter cultures of the captured cancer cells were also demonstrated.
AB - This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane "slot" filter. More than 90% viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase activity measurement of a single cancer cell taken from the filter after capture was proven. The on-filter and off-filter cultures of the captured cancer cells were also demonstrated.
UR - http://www.scopus.com/inward/record.url?scp=77952771018&partnerID=8YFLogxK
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U2 - 10.1109/MEMSYS.2010.5442361
DO - 10.1109/MEMSYS.2010.5442361
M3 - Conference contribution
AN - SCOPUS:77952771018
SN - 9781424457649
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 935
EP - 938
BT - MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
T2 - 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
Y2 - 24 January 2010 through 28 January 2010
ER -