Pasteurella multocida gene expression in response to iron limitation

M. L. Paustian, B. J. May, Vivek Kapur

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Pasteurella multocida is the causative agent of a wide range of diseases in avian and mammalian hosts. Gene expression in response to low iron conditions was analyzed in P. multocida using whole-genome microarrays. The analysis shows that the expression of genes involved in energy metabolism and electron transport generally decreased 2.1- to 6-fold while that of genes used for iron binding and transport increased 2.1- to 7.7-fold in P. multocida during the first 2 h of growth under iron-limiting conditions compared with controls. Notably, 27% of the genes with significantly altered expression had no known function, illustrating the limitations of using publicly available databases to identify genes involved in microbial metabolism and pathogenesis. Taken together, the results of our investigations demonstrate the utility of whole-genome microarray analyses for the identification of genes with altered expression profiles during varying growth conditions and provide a framework for the detailed analysis of the molecular mechanisms of iron acquisition and metabolism in P. multocida and other gram-negative bacteria.

Original languageEnglish (US)
Pages (from-to)4109-4115
Number of pages7
JournalInfection and Immunity
Volume69
Issue number6
DOIs
StatePublished - Jun 11 2001

Fingerprint

Pasteurella multocida
Iron
Gene Expression
Genes
Bird Diseases
Genome
Microarray Analysis
Growth
Electron Transport
Gram-Negative Bacteria
Energy Metabolism
Databases

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Paustian, M. L. ; May, B. J. ; Kapur, Vivek. / Pasteurella multocida gene expression in response to iron limitation. In: Infection and Immunity. 2001 ; Vol. 69, No. 6. pp. 4109-4115.
@article{e189b0c612094913857648f39db86e0d,
title = "Pasteurella multocida gene expression in response to iron limitation",
abstract = "Pasteurella multocida is the causative agent of a wide range of diseases in avian and mammalian hosts. Gene expression in response to low iron conditions was analyzed in P. multocida using whole-genome microarrays. The analysis shows that the expression of genes involved in energy metabolism and electron transport generally decreased 2.1- to 6-fold while that of genes used for iron binding and transport increased 2.1- to 7.7-fold in P. multocida during the first 2 h of growth under iron-limiting conditions compared with controls. Notably, 27{\%} of the genes with significantly altered expression had no known function, illustrating the limitations of using publicly available databases to identify genes involved in microbial metabolism and pathogenesis. Taken together, the results of our investigations demonstrate the utility of whole-genome microarray analyses for the identification of genes with altered expression profiles during varying growth conditions and provide a framework for the detailed analysis of the molecular mechanisms of iron acquisition and metabolism in P. multocida and other gram-negative bacteria.",
author = "Paustian, {M. L.} and May, {B. J.} and Vivek Kapur",
year = "2001",
month = "6",
day = "11",
doi = "10.1128/IAI.69.6.4109-4115.2001",
language = "English (US)",
volume = "69",
pages = "4109--4115",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "6",

}

Pasteurella multocida gene expression in response to iron limitation. / Paustian, M. L.; May, B. J.; Kapur, Vivek.

In: Infection and Immunity, Vol. 69, No. 6, 11.06.2001, p. 4109-4115.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Pasteurella multocida gene expression in response to iron limitation

AU - Paustian, M. L.

AU - May, B. J.

AU - Kapur, Vivek

PY - 2001/6/11

Y1 - 2001/6/11

N2 - Pasteurella multocida is the causative agent of a wide range of diseases in avian and mammalian hosts. Gene expression in response to low iron conditions was analyzed in P. multocida using whole-genome microarrays. The analysis shows that the expression of genes involved in energy metabolism and electron transport generally decreased 2.1- to 6-fold while that of genes used for iron binding and transport increased 2.1- to 7.7-fold in P. multocida during the first 2 h of growth under iron-limiting conditions compared with controls. Notably, 27% of the genes with significantly altered expression had no known function, illustrating the limitations of using publicly available databases to identify genes involved in microbial metabolism and pathogenesis. Taken together, the results of our investigations demonstrate the utility of whole-genome microarray analyses for the identification of genes with altered expression profiles during varying growth conditions and provide a framework for the detailed analysis of the molecular mechanisms of iron acquisition and metabolism in P. multocida and other gram-negative bacteria.

AB - Pasteurella multocida is the causative agent of a wide range of diseases in avian and mammalian hosts. Gene expression in response to low iron conditions was analyzed in P. multocida using whole-genome microarrays. The analysis shows that the expression of genes involved in energy metabolism and electron transport generally decreased 2.1- to 6-fold while that of genes used for iron binding and transport increased 2.1- to 7.7-fold in P. multocida during the first 2 h of growth under iron-limiting conditions compared with controls. Notably, 27% of the genes with significantly altered expression had no known function, illustrating the limitations of using publicly available databases to identify genes involved in microbial metabolism and pathogenesis. Taken together, the results of our investigations demonstrate the utility of whole-genome microarray analyses for the identification of genes with altered expression profiles during varying growth conditions and provide a framework for the detailed analysis of the molecular mechanisms of iron acquisition and metabolism in P. multocida and other gram-negative bacteria.

UR - http://www.scopus.com/inward/record.url?scp=0035010085&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035010085&partnerID=8YFLogxK

U2 - 10.1128/IAI.69.6.4109-4115.2001

DO - 10.1128/IAI.69.6.4109-4115.2001

M3 - Article

C2 - 11349083

AN - SCOPUS:0035010085

VL - 69

SP - 4109

EP - 4115

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 6

ER -