Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells

Yiming Zhang, Sisi Zheng, Yubin Zhou, Donald Gill, Youjun Wang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Induced by the depletion of ER calcium store, the calcium influx through calcium release-activated calcium (CRAC) channels is an ubiquitous way of Ca 2+ influx for most cell types. This process is mediated by STIM protein, ER calcium sensor, and PM localized Orai calcium channels. Biophysical characterization of this STIM-Orai-mediated current, or I CRAC , with whole-cell patch-clamp technique is essential for revealing the molecular mechanisms underlying the process of STIM-Orai activation or modulation. Here we describe one commonly used procedure of monitoring CRAC activity with whole-cell patch-clamp technique.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages1-16
Number of pages16
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1843
ISSN (Print)1064-3745

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Zhang, Y., Zheng, S., Zhou, Y., Gill, D., & Wang, Y. (2018). Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells. In Methods in Molecular Biology (pp. 1-16). (Methods in Molecular Biology; Vol. 1843). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8704-7_1