Pathogen-Mediated Tritrophic Interactions

Baculovirus-Challenged Caterpillars Induce Higher Plant Defenses than Healthy Caterpillars

Qinjian Pan, Ikkei Shikano, Kelli Hoover, Tong Xian Liu, Gary Felton

Research output: Contribution to journalArticle

Abstract

Although the tritrophic interactions of plants, insect herbivores and their natural enemies have been intensely studied for several decades, the roles of entomopathogens in their indirect modulation of plant-insect relationships is still unclear. Here, we employed a sublethal dose of a baculovirus with a relatively broad host range (AcMNPV) to explore if feeding by baculovirus-challenged Helicoverpa zea caterpillars induces direct defenses in the tomato plant. We examined induction of plant defenses following feeding by H. zea, including tomato plants fed on by healthy caterpillars, AcMNPV-challenged caterpillars, or undamaged controls, and subsequently compared the transcript levels of defense related proteins (i.e., trypsin proteinase inhibitors, peroxidase and polyphenol oxidase) and other defense genes (i.e., proteinase inhibitor II and cysteine proteinase inhibitor) from these plants, in addition to comparing caterpillar relative growth rates. As a result, AcMNPV-challenged caterpillars induced the highest plant anti-herbivore defenses. We examined several elicitors and effectors in the secretions of these caterpillars (i.e., glucose oxidase, phospholipase C, and ATPase hydrolysis), which surprisingly did not differ between treatments. Hence, we suggest that the greater induction of plant defenses by the virus-challenged caterpillars may be due to differences in the amount of these secretions deposited during feeding or to some other unknown factor(s).

Original languageEnglish (US)
Pages (from-to)515-524
Number of pages10
JournalJournal of Chemical Ecology
Volume45
Issue number5-6
DOIs
StatePublished - Jun 15 2019

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tritrophic interaction
tritrophic interactions
plant defense
Baculoviridae
caterpillar
Pathogens
insect larvae
pathogen
pathogens
Peptide Hydrolases
Autographa californica multiple nucleopolyhedrovirus
Cysteine Proteinase Inhibitors
Catechol Oxidase
Glucose Oxidase
inhibitor
Type C Phospholipases
Herbivory
Viruses
Trypsin
Peroxidase

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

Cite this

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title = "Pathogen-Mediated Tritrophic Interactions: Baculovirus-Challenged Caterpillars Induce Higher Plant Defenses than Healthy Caterpillars",
abstract = "Although the tritrophic interactions of plants, insect herbivores and their natural enemies have been intensely studied for several decades, the roles of entomopathogens in their indirect modulation of plant-insect relationships is still unclear. Here, we employed a sublethal dose of a baculovirus with a relatively broad host range (AcMNPV) to explore if feeding by baculovirus-challenged Helicoverpa zea caterpillars induces direct defenses in the tomato plant. We examined induction of plant defenses following feeding by H. zea, including tomato plants fed on by healthy caterpillars, AcMNPV-challenged caterpillars, or undamaged controls, and subsequently compared the transcript levels of defense related proteins (i.e., trypsin proteinase inhibitors, peroxidase and polyphenol oxidase) and other defense genes (i.e., proteinase inhibitor II and cysteine proteinase inhibitor) from these plants, in addition to comparing caterpillar relative growth rates. As a result, AcMNPV-challenged caterpillars induced the highest plant anti-herbivore defenses. We examined several elicitors and effectors in the secretions of these caterpillars (i.e., glucose oxidase, phospholipase C, and ATPase hydrolysis), which surprisingly did not differ between treatments. Hence, we suggest that the greater induction of plant defenses by the virus-challenged caterpillars may be due to differences in the amount of these secretions deposited during feeding or to some other unknown factor(s).",
author = "Qinjian Pan and Ikkei Shikano and Kelli Hoover and Liu, {Tong Xian} and Gary Felton",
year = "2019",
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T2 - Baculovirus-Challenged Caterpillars Induce Higher Plant Defenses than Healthy Caterpillars

AU - Pan, Qinjian

AU - Shikano, Ikkei

AU - Hoover, Kelli

AU - Liu, Tong Xian

AU - Felton, Gary

PY - 2019/6/15

Y1 - 2019/6/15

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