Pathogenesis of a model gammaherpesvirus in a natural host

David J. Hughes, Anja Kipar, Jeffery Sample, James P. Stewart

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Murine gammaherpesvirus 68 (MHV-68) infection of laboratory mice (Mus musculus) is an established model of gammaherpesvirus pathogenesis. The fact that M. musculus is not a host in the wild prompted us to reassess MHV-68 infection in wood mice (Apodemus sylvaticus), a natural host. Here, we report significant differences in MHV-68 infection in the two species: (i) following intranasal inoculation, MHV-68 replicated in the lungs of wood mice to levels approximately 3 log units lower than in BALB/c mice; (ii) in BALB/c mice, virus replication in alveolar epithelial cells was accompanied by a diffuse, T-cell-dominated interstitial pneumonitis, whereas in wood mice it was restricted to focal granulomatous infiltrations; (iii) within wood mice, latently infected lymphocytes were abundant in inducible bronchus-associated lymphoid tissue that was not apparent in BALB/c mice; (iv) splenic latency was established in both species, but well-delineated secondary follicles with germinal centers were present in wood mice, while only poorly delineated follicles were seen in BALB/c mice; and, perhaps as a consequence, (v) production of neutralizing antibody was significantly higher in wood mice. These differences highlight the value of this animal model in the study of MHV-68 pathogenesis.

Original languageEnglish (US)
Pages (from-to)3949-3961
Number of pages13
JournalJournal of virology
Volume84
Issue number8
DOIs
StatePublished - Apr 1 2010

Fingerprint

Murid herpesvirus 4
Apodemus
pathogenesis
mice
Mus musculus
infection
Apodemus sylvaticus
bronchi
virus replication
neutralizing antibodies
pneumonia
epithelial cells
lymphocytes
T-lymphocytes
animal models
lungs
Infection
Murinae
Alveolar Epithelial Cells
Germinal Center

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Hughes, David J. ; Kipar, Anja ; Sample, Jeffery ; Stewart, James P. / Pathogenesis of a model gammaherpesvirus in a natural host. In: Journal of virology. 2010 ; Vol. 84, No. 8. pp. 3949-3961.
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Pathogenesis of a model gammaherpesvirus in a natural host. / Hughes, David J.; Kipar, Anja; Sample, Jeffery; Stewart, James P.

In: Journal of virology, Vol. 84, No. 8, 01.04.2010, p. 3949-3961.

Research output: Contribution to journalArticle

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