Pathogenicity of mutants of Erwinia carotovora subsp. carotovora deficient in aerobactin and catecholate siderophore production

Carolee Theresa Bull, Susan R. Carnegie, Joyce E. Loper

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Erwinia carotovora subsp. carotovora strain W3C105 produces the hydroxamate siderophore aerobactin and a catecholate metabolite, which previously was not known to function in iron acquisition by the phytopathogen. A region containing catechol biosynthesis genes (cbsEA*) of strain W3C105 complements EntA- and EntE- mutants of Escherichia coli, which are deficient in biosynthesis of the catecholate siderophore coli, which are deficient in biosynthesis of the catecholate siderophore woth Tn3-Spice complemented either the EntA- or the EntE- mutant of E. coli but not both mutants. Two of the plasmids, in which insertions of Tn3-Spice generated transcriptional fusions of the promoterless ice nucleation reporter gene (inaZ) to a cbs promoter(s), conferred iron-regulated ice nucleation activity on E. coli. One of the Tn3-Spice insertions was introfuced into the genome of W3C105 by marker-exchange mutagenesis to generate a mutant deficient in catechol biosynthesis (Cbs-). Introduction of the insertion into a previously-derived mutant of W3C105 deficient in aerobactin biosynthesis (Iuc-) resulted in a double mutant that produced neither the catechol nor aerobactin (Cbs- Iuc-). A catechol-producing mutant (Cbs* Iuc-) grew on a minimal medium containing higher concentrations of the iron-chelator 2,2′-dipyridyl than did a mutant producing neither siderophore (Cbs- Iuc-). demonstrating that the catechol functions as a siderophore in strain W3C105. Wild-type W3C105 and an aerobactin-producing mutant (Cbs- Iuc*), however, grew on a minimal medium containing higner concenteations of 2,2′-dipyridyl that did a mutant producing only the catechol (Cbs* Iuc-), indicating that aerobactin was superior to the catecholate siderophore in competing with 2,2′-dipyridyl for iron. Mutants deficient in the production of either or both siderophores did not differ from the wild-type strain in the capacity to macerate potato tuber tissue or cause aerial stem rot of potato.

Original languageEnglish (US)
Pages (from-to)260-266
Number of pages7
JournalPhytopathology
Volume86
Issue number3
DOIs
StatePublished - Jan 1 1996

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Pectobacterium carotovorum subsp. carotovorum
siderophores
catechol
pathogenicity
mutants
biosynthesis
spices
iron
ice nucleation
Escherichia coli
potatoes
stem rot
chelating agents
reporter genes
mutagenesis
plant pathogens
plasmids
complement
tubers

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

@article{15a95a3d3f5b492f895c6accf7c7513e,
title = "Pathogenicity of mutants of Erwinia carotovora subsp. carotovora deficient in aerobactin and catecholate siderophore production",
abstract = "Erwinia carotovora subsp. carotovora strain W3C105 produces the hydroxamate siderophore aerobactin and a catecholate metabolite, which previously was not known to function in iron acquisition by the phytopathogen. A region containing catechol biosynthesis genes (cbsEA*) of strain W3C105 complements EntA- and EntE- mutants of Escherichia coli, which are deficient in biosynthesis of the catecholate siderophore coli, which are deficient in biosynthesis of the catecholate siderophore woth Tn3-Spice complemented either the EntA- or the EntE- mutant of E. coli but not both mutants. Two of the plasmids, in which insertions of Tn3-Spice generated transcriptional fusions of the promoterless ice nucleation reporter gene (inaZ) to a cbs promoter(s), conferred iron-regulated ice nucleation activity on E. coli. One of the Tn3-Spice insertions was introfuced into the genome of W3C105 by marker-exchange mutagenesis to generate a mutant deficient in catechol biosynthesis (Cbs-). Introduction of the insertion into a previously-derived mutant of W3C105 deficient in aerobactin biosynthesis (Iuc-) resulted in a double mutant that produced neither the catechol nor aerobactin (Cbs- Iuc-). A catechol-producing mutant (Cbs* Iuc-) grew on a minimal medium containing higher concentrations of the iron-chelator 2,2′-dipyridyl than did a mutant producing neither siderophore (Cbs- Iuc-). demonstrating that the catechol functions as a siderophore in strain W3C105. Wild-type W3C105 and an aerobactin-producing mutant (Cbs- Iuc*), however, grew on a minimal medium containing higner concenteations of 2,2′-dipyridyl that did a mutant producing only the catechol (Cbs* Iuc-), indicating that aerobactin was superior to the catecholate siderophore in competing with 2,2′-dipyridyl for iron. Mutants deficient in the production of either or both siderophores did not differ from the wild-type strain in the capacity to macerate potato tuber tissue or cause aerial stem rot of potato.",
author = "Bull, {Carolee Theresa} and Carnegie, {Susan R.} and Loper, {Joyce E.}",
year = "1996",
month = "1",
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doi = "10.1094/Phyto-86-260",
language = "English (US)",
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journal = "Phytopathology",
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}

Pathogenicity of mutants of Erwinia carotovora subsp. carotovora deficient in aerobactin and catecholate siderophore production. / Bull, Carolee Theresa; Carnegie, Susan R.; Loper, Joyce E.

In: Phytopathology, Vol. 86, No. 3, 01.01.1996, p. 260-266.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Pathogenicity of mutants of Erwinia carotovora subsp. carotovora deficient in aerobactin and catecholate siderophore production

AU - Bull, Carolee Theresa

AU - Carnegie, Susan R.

AU - Loper, Joyce E.

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N2 - Erwinia carotovora subsp. carotovora strain W3C105 produces the hydroxamate siderophore aerobactin and a catecholate metabolite, which previously was not known to function in iron acquisition by the phytopathogen. A region containing catechol biosynthesis genes (cbsEA*) of strain W3C105 complements EntA- and EntE- mutants of Escherichia coli, which are deficient in biosynthesis of the catecholate siderophore coli, which are deficient in biosynthesis of the catecholate siderophore woth Tn3-Spice complemented either the EntA- or the EntE- mutant of E. coli but not both mutants. Two of the plasmids, in which insertions of Tn3-Spice generated transcriptional fusions of the promoterless ice nucleation reporter gene (inaZ) to a cbs promoter(s), conferred iron-regulated ice nucleation activity on E. coli. One of the Tn3-Spice insertions was introfuced into the genome of W3C105 by marker-exchange mutagenesis to generate a mutant deficient in catechol biosynthesis (Cbs-). Introduction of the insertion into a previously-derived mutant of W3C105 deficient in aerobactin biosynthesis (Iuc-) resulted in a double mutant that produced neither the catechol nor aerobactin (Cbs- Iuc-). A catechol-producing mutant (Cbs* Iuc-) grew on a minimal medium containing higher concentrations of the iron-chelator 2,2′-dipyridyl than did a mutant producing neither siderophore (Cbs- Iuc-). demonstrating that the catechol functions as a siderophore in strain W3C105. Wild-type W3C105 and an aerobactin-producing mutant (Cbs- Iuc*), however, grew on a minimal medium containing higner concenteations of 2,2′-dipyridyl that did a mutant producing only the catechol (Cbs* Iuc-), indicating that aerobactin was superior to the catecholate siderophore in competing with 2,2′-dipyridyl for iron. Mutants deficient in the production of either or both siderophores did not differ from the wild-type strain in the capacity to macerate potato tuber tissue or cause aerial stem rot of potato.

AB - Erwinia carotovora subsp. carotovora strain W3C105 produces the hydroxamate siderophore aerobactin and a catecholate metabolite, which previously was not known to function in iron acquisition by the phytopathogen. A region containing catechol biosynthesis genes (cbsEA*) of strain W3C105 complements EntA- and EntE- mutants of Escherichia coli, which are deficient in biosynthesis of the catecholate siderophore coli, which are deficient in biosynthesis of the catecholate siderophore woth Tn3-Spice complemented either the EntA- or the EntE- mutant of E. coli but not both mutants. Two of the plasmids, in which insertions of Tn3-Spice generated transcriptional fusions of the promoterless ice nucleation reporter gene (inaZ) to a cbs promoter(s), conferred iron-regulated ice nucleation activity on E. coli. One of the Tn3-Spice insertions was introfuced into the genome of W3C105 by marker-exchange mutagenesis to generate a mutant deficient in catechol biosynthesis (Cbs-). Introduction of the insertion into a previously-derived mutant of W3C105 deficient in aerobactin biosynthesis (Iuc-) resulted in a double mutant that produced neither the catechol nor aerobactin (Cbs- Iuc-). A catechol-producing mutant (Cbs* Iuc-) grew on a minimal medium containing higher concentrations of the iron-chelator 2,2′-dipyridyl than did a mutant producing neither siderophore (Cbs- Iuc-). demonstrating that the catechol functions as a siderophore in strain W3C105. Wild-type W3C105 and an aerobactin-producing mutant (Cbs- Iuc*), however, grew on a minimal medium containing higner concenteations of 2,2′-dipyridyl that did a mutant producing only the catechol (Cbs* Iuc-), indicating that aerobactin was superior to the catecholate siderophore in competing with 2,2′-dipyridyl for iron. Mutants deficient in the production of either or both siderophores did not differ from the wild-type strain in the capacity to macerate potato tuber tissue or cause aerial stem rot of potato.

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