Pathogenicity of mutants of Erwinia carotovora subsp. carotovora deficient in aerobactin and catecholate siderophore production

Carolee Theresa Bull, Susan R. Carnegie, Joyce E. Loper

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12 Scopus citations

Abstract

Erwinia carotovora subsp. carotovora strain W3C105 produces the hydroxamate siderophore aerobactin and a catecholate metabolite, which previously was not known to function in iron acquisition by the phytopathogen. A region containing catechol biosynthesis genes (cbsEA*) of strain W3C105 complements EntA- and EntE- mutants of Escherichia coli, which are deficient in biosynthesis of the catecholate siderophore coli, which are deficient in biosynthesis of the catecholate siderophore woth Tn3-Spice complemented either the EntA- or the EntE- mutant of E. coli but not both mutants. Two of the plasmids, in which insertions of Tn3-Spice generated transcriptional fusions of the promoterless ice nucleation reporter gene (inaZ) to a cbs promoter(s), conferred iron-regulated ice nucleation activity on E. coli. One of the Tn3-Spice insertions was introfuced into the genome of W3C105 by marker-exchange mutagenesis to generate a mutant deficient in catechol biosynthesis (Cbs-). Introduction of the insertion into a previously-derived mutant of W3C105 deficient in aerobactin biosynthesis (Iuc-) resulted in a double mutant that produced neither the catechol nor aerobactin (Cbs- Iuc-). A catechol-producing mutant (Cbs* Iuc-) grew on a minimal medium containing higher concentrations of the iron-chelator 2,2′-dipyridyl than did a mutant producing neither siderophore (Cbs- Iuc-). demonstrating that the catechol functions as a siderophore in strain W3C105. Wild-type W3C105 and an aerobactin-producing mutant (Cbs- Iuc*), however, grew on a minimal medium containing higner concenteations of 2,2′-dipyridyl that did a mutant producing only the catechol (Cbs* Iuc-), indicating that aerobactin was superior to the catecholate siderophore in competing with 2,2′-dipyridyl for iron. Mutants deficient in the production of either or both siderophores did not differ from the wild-type strain in the capacity to macerate potato tuber tissue or cause aerial stem rot of potato.

Original languageEnglish (US)
Pages (from-to)260-266
Number of pages7
JournalPhytopathology
Volume86
Issue number3
DOIs
StatePublished - Jan 1 1996

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

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