Patronin-mediated minus end growth is required for dendritic microtubule polarity

Chengye Feng, Pankajam Thyagarajan, Matthew Shorey, Dylan Y. Seebold, Alexis T. Weiner, Richard M. Albertson, Kavitha S. Rao, Alvaro Sagasti, Daniel J. Goetschius, Melissa M. Rolls

Research output: Contribution to journalArticle

Abstract

Microtubule minus ends are thought to be stable in cells. Surprisingly, in Drosophila and zebrafish neurons, we observed persistent minus end growth, with runs lasting over 10 min. In Drosophila, extended minus end growth depended on Patronin, and Patronin reduction disrupted dendritic minus-end-out polarity. In fly dendrites, microtubule nucleation sites localize at dendrite branch points. Therefore, we hypothesized minus end growth might be particularly important beyond branch points. Distal dendrites have mixed polarity, and reduction of Patronin lowered the number of minus-end-out microtubules. More strikingly, extra Patronin made terminal dendrites almost completely minus-end-out, indicating low Patronin normally limits minus-end-out microtubules. To determine whether minus end growth populated new dendrites with microtubules, we analyzed dendrite development and regeneration. Minus ends extended into growing dendrites in the presence of Patronin. In sum, our data suggest that Patronin facilitates sustained microtubule minus end growth, which is critical for populating dendrites with minus-end-out microtubules.

Original languageEnglish (US)
Pages (from-to)2309-2328
Number of pages20
JournalJournal of Cell Biology
Volume218
Issue number7
DOIs
StatePublished - Jan 1 2019

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Dendrites
Microtubules
Growth
Drosophila
Zebrafish
Diptera
Regeneration
Neurons

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Feng, C., Thyagarajan, P., Shorey, M., Seebold, D. Y., Weiner, A. T., Albertson, R. M., ... Rolls, M. M. (2019). Patronin-mediated minus end growth is required for dendritic microtubule polarity. Journal of Cell Biology, 218(7), 2309-2328. https://doi.org/10.1083/jcb.201810155
Feng, Chengye ; Thyagarajan, Pankajam ; Shorey, Matthew ; Seebold, Dylan Y. ; Weiner, Alexis T. ; Albertson, Richard M. ; Rao, Kavitha S. ; Sagasti, Alvaro ; Goetschius, Daniel J. ; Rolls, Melissa M. / Patronin-mediated minus end growth is required for dendritic microtubule polarity. In: Journal of Cell Biology. 2019 ; Vol. 218, No. 7. pp. 2309-2328.
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Feng, C, Thyagarajan, P, Shorey, M, Seebold, DY, Weiner, AT, Albertson, RM, Rao, KS, Sagasti, A, Goetschius, DJ & Rolls, MM 2019, 'Patronin-mediated minus end growth is required for dendritic microtubule polarity', Journal of Cell Biology, vol. 218, no. 7, pp. 2309-2328. https://doi.org/10.1083/jcb.201810155

Patronin-mediated minus end growth is required for dendritic microtubule polarity. / Feng, Chengye; Thyagarajan, Pankajam; Shorey, Matthew; Seebold, Dylan Y.; Weiner, Alexis T.; Albertson, Richard M.; Rao, Kavitha S.; Sagasti, Alvaro; Goetschius, Daniel J.; Rolls, Melissa M.

In: Journal of Cell Biology, Vol. 218, No. 7, 01.01.2019, p. 2309-2328.

Research output: Contribution to journalArticle

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T1 - Patronin-mediated minus end growth is required for dendritic microtubule polarity

AU - Feng, Chengye

AU - Thyagarajan, Pankajam

AU - Shorey, Matthew

AU - Seebold, Dylan Y.

AU - Weiner, Alexis T.

AU - Albertson, Richard M.

AU - Rao, Kavitha S.

AU - Sagasti, Alvaro

AU - Goetschius, Daniel J.

AU - Rolls, Melissa M.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Microtubule minus ends are thought to be stable in cells. Surprisingly, in Drosophila and zebrafish neurons, we observed persistent minus end growth, with runs lasting over 10 min. In Drosophila, extended minus end growth depended on Patronin, and Patronin reduction disrupted dendritic minus-end-out polarity. In fly dendrites, microtubule nucleation sites localize at dendrite branch points. Therefore, we hypothesized minus end growth might be particularly important beyond branch points. Distal dendrites have mixed polarity, and reduction of Patronin lowered the number of minus-end-out microtubules. More strikingly, extra Patronin made terminal dendrites almost completely minus-end-out, indicating low Patronin normally limits minus-end-out microtubules. To determine whether minus end growth populated new dendrites with microtubules, we analyzed dendrite development and regeneration. Minus ends extended into growing dendrites in the presence of Patronin. In sum, our data suggest that Patronin facilitates sustained microtubule minus end growth, which is critical for populating dendrites with minus-end-out microtubules.

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Feng C, Thyagarajan P, Shorey M, Seebold DY, Weiner AT, Albertson RM et al. Patronin-mediated minus end growth is required for dendritic microtubule polarity. Journal of Cell Biology. 2019 Jan 1;218(7):2309-2328. https://doi.org/10.1083/jcb.201810155