Patterning high surface area silica with lysozyme: Adsorption kinetics, fluorescence quenching, and protein readsorption studies to evaluate the templated surface

Rachel M. Greer, Brittni A. Scruggs, R. Alan May, Bert D. Chandler

Research output: Contribution to journalArticlepeer-review

Abstract

A method was developed for using an inexpensive and widely available protein, hen egg white lysozyme, as a patterning agent for commercial high surface area silicas. The basic patterning methodology involved spontaneous adsorption of the protein from aqueous solution, alkylation of the uncovered surface with an alkylsiloxane, and protein desorption in a slightly alkaline solution of morpholine. Adsorption kinetic studies using Bradford assays assisted in determining protein deposition conditions. These studies were generally consistent with results on more planar silica surfaces and indicated that the protein quickly and strongly adsorbs along its long axis at low surface coverages. A modified fluorescence resonance energy transfer (FRET) technique was developed and employed to evaluate protein spacing. This technique showed that the proteins are well dispersed at low coverages. Readsorption experiments show that the templated regions are robust, retaining the size and shape of the original protein templates.

Original languageEnglish (US)
Pages (from-to)7161-7168
Number of pages8
JournalLangmuir
Volume25
Issue number12
DOIs
StatePublished - Jun 16 2009

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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