Patterns of diversity in populations of the turfgrass pathogen Colletotrichum cereale as revealed by transposon fingerprint profiles

Jo Anne Crouch, Bernadette M. Glasheen, Wakar Uddin, Bruce B. Clarke, Bradley I. Hillman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Anthracnose disease of cool-season turfgrasses, caused by the fungus Colletotrichum cereale, has recently emerged as one of the most significant pathogens of Poa annua. Here we investigated the utility of four repetitive transposable elements as molecular markers for the analysis of C. cereale populations. Southern blot hybridization analysis revealed lineage-specific polymorphisms and distribution patterns for these transposons. Comparative phylogenetic analysis of three nonrepetitive protein coding DNA sequences against the transposon restriction fragment length polymorphisms indicated that the transposon sequences have similar evolutionary histories to those found in the sampled C. cereale population, despite the alteration of several transposon copies by repeat-induced point mutation. The variability and ubiquity of the Ccret2A15 transposon in C. cereale genomes suggest that this element could be used as a reliable DNA marker to discriminate between lineages of the fungus, identify hybrid genotypes, and analyze genetic diversity in populations of this turfgrass pathogen.

Original languageEnglish (US)
Pages (from-to)1203-1210
Number of pages8
JournalCrop Science
Volume48
Issue number3
DOIs
StatePublished - May 1 2008

Fingerprint

Colletotrichum
turf grasses
transposons
pathogens
Poa annua
fungi
genetic markers
anthracnose
point mutation
mutagenesis
Southern blotting
restriction fragment length polymorphism
hybridization
genetic polymorphism
nucleotide sequences
genetic variation
history
genome
genotype
phylogeny

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science

Cite this

Crouch, Jo Anne ; Glasheen, Bernadette M. ; Uddin, Wakar ; Clarke, Bruce B. ; Hillman, Bradley I. / Patterns of diversity in populations of the turfgrass pathogen Colletotrichum cereale as revealed by transposon fingerprint profiles. In: Crop Science. 2008 ; Vol. 48, No. 3. pp. 1203-1210.
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Patterns of diversity in populations of the turfgrass pathogen Colletotrichum cereale as revealed by transposon fingerprint profiles. / Crouch, Jo Anne; Glasheen, Bernadette M.; Uddin, Wakar; Clarke, Bruce B.; Hillman, Bradley I.

In: Crop Science, Vol. 48, No. 3, 01.05.2008, p. 1203-1210.

Research output: Contribution to journalArticle

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