TY - JOUR
T1 - Pax6 regulates Tbr1 and Tbr2 expressions in olfactory bulb mitral cells
AU - Imamura, Fumiaki
AU - Greer, Charles A.
N1 - Funding Information:
We thank Drs. Kenneth Kwan, Mandy Lam, and Nenad Šestan for the guidance in the use of in utero electroporation and Dr. Yoshihiro Yoshihara for the antibody to Tbx21. We also thank Drs. Arie Mobley and Diego Rodriguez-Gil for the critical reading of the manuscript and all the members of Greer laboratory for technical assistance and discussion. The antibody to Pax6 developed by Atsushi Kawakami was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the US National Institute of Child Health and Human Development and maintained by the Department of Biology, University of Iowa. This work was supported by NIH grants DC000210 (C.A.G.) and DC011134 (F.I.).
PY - 2013/5
Y1 - 2013/5
N2 - Tracking olfactory bulb mitral cell development with BrdU labeling, we find that mitral cells are generated from Pax6. + radial glial cells in the ventricular zone of the embryonic olfactory bulb. Unlike cortical projection neurons, postmitotic mitral cell precursors express both Tbr1 and Tbr2. Our tracking experiments revealed that down-regulation of Pax6 preceded up-regulation of Tbrs, and that Tbr1 emerged earlier than Tbr2. Using in utero electroporation, we also show that Pax6 negatively regulates the expression of Tbr1 and Tbr2 in postmitotic mitral cell precursors. Exogenous expression of Pax6 in embryonic olfactory bulb postmitotic precursors decreased the number of cells that progressed to a mitral cell fate. In contrast, exogenous expression of Pax6 resulted in an increase of GABAergic and/or dopaminergic interneurons. These results indicate that Pax6 is a regulator of fate determination of precursor cells.
AB - Tracking olfactory bulb mitral cell development with BrdU labeling, we find that mitral cells are generated from Pax6. + radial glial cells in the ventricular zone of the embryonic olfactory bulb. Unlike cortical projection neurons, postmitotic mitral cell precursors express both Tbr1 and Tbr2. Our tracking experiments revealed that down-regulation of Pax6 preceded up-regulation of Tbrs, and that Tbr1 emerged earlier than Tbr2. Using in utero electroporation, we also show that Pax6 negatively regulates the expression of Tbr1 and Tbr2 in postmitotic mitral cell precursors. Exogenous expression of Pax6 in embryonic olfactory bulb postmitotic precursors decreased the number of cells that progressed to a mitral cell fate. In contrast, exogenous expression of Pax6 resulted in an increase of GABAergic and/or dopaminergic interneurons. These results indicate that Pax6 is a regulator of fate determination of precursor cells.
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U2 - 10.1016/j.mcn.2013.01.002
DO - 10.1016/j.mcn.2013.01.002
M3 - Article
C2 - 23353076
AN - SCOPUS:84873722937
VL - 54
SP - 58
EP - 70
JO - Molecular and Cellular Neurosciences
JF - Molecular and Cellular Neurosciences
SN - 1044-7431
ER -