PCNA interacts with hHusl/hRad9 in response to DNA damage and replication inhibition

Kiyoshi Komatsu, Walker Wharton, Haiying Hang, Chun Wu, Sujay Singh, Howard B. Lieberman, W. J. Pledger, Hong-Gang Wang

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

The hHus1 and several hRad proteins are involved in the control of DNA integrity checkpoints, although the mechanisms underlying these processes are unknown. Using a yeast two-hybrid system to detect protein-protein interactions, we found that human proliferating cell nuclear antigen (PCNA), a protein known to function in both DNA replication and repair, interacts with the human checkpoint-related protein Hus1 (hHus1). In human skin fibroblast cells, exposure to ionizing radiation of hydroxyurea triggers translocation of hHus1 from the cytosol to the nucleus, where it associates with PCNA as well as another checkpoint protein, hRad9. This nuclear translocation and the complex formation or hHus1 with PCNA and hRad9 correlate closely with changes in cell cycle distribution in response to radiation exposure. These results suggest that this multi-protein complex may be important for coordinating cell-cycle progression, DNA replication and repair of damaged DNA.

Original languageEnglish (US)
Pages (from-to)5291-5297
Number of pages7
JournalOncogene
Volume19
Issue number46
DOIs
StatePublished - Nov 2 2000

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Komatsu, K., Wharton, W., Hang, H., Wu, C., Singh, S., Lieberman, H. B., Pledger, W. J., & Wang, H-G. (2000). PCNA interacts with hHusl/hRad9 in response to DNA damage and replication inhibition. Oncogene, 19(46), 5291-5297. https://doi.org/10.1038/sj.onc.1203901