Pcr-based strategies used for tile identification ant) differentiation of typical strains of erwinia amylovora

Eric Wayne Brown, R. M. Davis, T. Van Der Zwet

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Several methods are currently available for the identification and differentiation of Erwinia arnylovora, the bacterium which causes fire blight. Historically, many laboratories have relied upon diagnostic medias or subtle differences in nutritional requirements to identify and distinguish plant pathogenic bacterial species. However, diagnostic protocols exploiting recent advances in molecular biology have become readily available to the phytobacteriologist. Specifically, the polymerase chain reaction (PCR) affords a rapid and reliable approach to identify and differentiate strains of E. amylovora as they are isolated from novel fire blight outbreaks. Three techniques, in particular, have been developed either specifically for use with E. amylovora or have been applied to the fire blight bacterium after being used to characterize other veterinary or medically significant prokaryotic systems. These techniques have been designated as follows: i) pEA29 PCR, ii) 'ribotyping', and iii) REP PCR. Below, we discuss the backgrounds associated with these rapid molecular genetic techniques and present detailed protocols for the three techniques in a single unifying format.

Original languageEnglish (US)
Title of host publicationVIII International Workshop on Fire Blight
EditorsM.T. Momol, H. Saygili
Pages159-163
Number of pages5
StatePublished - Dec 1 1999

Publication series

NameActa Horticulturae
Volume489
ISSN (Print)0567-7572

Fingerprint

Erwinia amylovora
tiles
Formicidae
polymerase chain reaction
Erwinia
methodology
bacteria
DNA fingerprinting
nutrient requirements
molecular genetics
molecular biology

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Brown, E. W., Davis, R. M., & Van Der Zwet, T. (1999). Pcr-based strategies used for tile identification ant) differentiation of typical strains of erwinia amylovora. In M. T. Momol, & H. Saygili (Eds.), VIII International Workshop on Fire Blight (pp. 159-163). (Acta Horticulturae; Vol. 489).
Brown, Eric Wayne ; Davis, R. M. ; Van Der Zwet, T. / Pcr-based strategies used for tile identification ant) differentiation of typical strains of erwinia amylovora. VIII International Workshop on Fire Blight. editor / M.T. Momol ; H. Saygili. 1999. pp. 159-163 (Acta Horticulturae).
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Brown, EW, Davis, RM & Van Der Zwet, T 1999, Pcr-based strategies used for tile identification ant) differentiation of typical strains of erwinia amylovora. in MT Momol & H Saygili (eds), VIII International Workshop on Fire Blight. Acta Horticulturae, vol. 489, pp. 159-163.

Pcr-based strategies used for tile identification ant) differentiation of typical strains of erwinia amylovora. / Brown, Eric Wayne; Davis, R. M.; Van Der Zwet, T.

VIII International Workshop on Fire Blight. ed. / M.T. Momol; H. Saygili. 1999. p. 159-163 (Acta Horticulturae; Vol. 489).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Brown EW, Davis RM, Van Der Zwet T. Pcr-based strategies used for tile identification ant) differentiation of typical strains of erwinia amylovora. In Momol MT, Saygili H, editors, VIII International Workshop on Fire Blight. 1999. p. 159-163. (Acta Horticulturae).