PCR detection of lizard malaria parasites: Prevalence of Plasmodium infections with low-level parasitemia differs by site and season

Anne Marie Vardo-Zalik, Andrew R. Wargo, Jos J. Schall

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Plasmodium-specific polymerase chain reaction (PCR) primers allowed detection of infections with very low-level parasitemia for 3 species of malaria parasites infecting Anolis lizards at 2 Caribbean sites, Puerto Rico and Saba, Netherlands Antilles. A verification study, using a single-tube nested PCR to eliminate contamination, showed that infections as low as 1 parasite per millions of erythrocytes could be detected by amplifying a 673 bp fragment of the cytochrome b gene. Very low-level parasitemia infections, subpatent under the microscope, were common in A. sabanus on Saba sites, with no significant seasonal difference (31% of infections appearing uninfected by microscopic examination in summer were found infected by PCR, 38% in winter). At the Puerto Rico site, the subpatent infections were also common in A. gundlachi, but were more prevalent in winter (53%) than in summer (17%). A similar high frequency of subpatent infections is known from studies on human and bird malaria, but a previous PCR-based study on a temperate lizard malaria system found few such low-level infections. Differences in the prevalence of subpatent infections by site and season suggest transmission biology may select for distinct life history strategies by the parasite.

Original languageEnglish (US)
Pages (from-to)1509-1511
Number of pages3
JournalJournal of Parasitology
Volume91
Issue number6
DOIs
StatePublished - Dec 1 2005

Fingerprint

parasite prevalence
Lizards
Parasitemia
parasitemia
malaria
Plasmodium
lizard
polymerase chain reaction
Malaria
lizards
Parasites
parasites
Polymerase Chain Reaction
Infection
Netherlands Antilles
infection
Puerto Rico
parasite
Anolis
Cytochromes b

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

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title = "PCR detection of lizard malaria parasites: Prevalence of Plasmodium infections with low-level parasitemia differs by site and season",
abstract = "Plasmodium-specific polymerase chain reaction (PCR) primers allowed detection of infections with very low-level parasitemia for 3 species of malaria parasites infecting Anolis lizards at 2 Caribbean sites, Puerto Rico and Saba, Netherlands Antilles. A verification study, using a single-tube nested PCR to eliminate contamination, showed that infections as low as 1 parasite per millions of erythrocytes could be detected by amplifying a 673 bp fragment of the cytochrome b gene. Very low-level parasitemia infections, subpatent under the microscope, were common in A. sabanus on Saba sites, with no significant seasonal difference (31{\%} of infections appearing uninfected by microscopic examination in summer were found infected by PCR, 38{\%} in winter). At the Puerto Rico site, the subpatent infections were also common in A. gundlachi, but were more prevalent in winter (53{\%}) than in summer (17{\%}). A similar high frequency of subpatent infections is known from studies on human and bird malaria, but a previous PCR-based study on a temperate lizard malaria system found few such low-level infections. Differences in the prevalence of subpatent infections by site and season suggest transmission biology may select for distinct life history strategies by the parasite.",
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PCR detection of lizard malaria parasites : Prevalence of Plasmodium infections with low-level parasitemia differs by site and season. / Vardo-Zalik, Anne Marie; Wargo, Andrew R.; Schall, Jos J.

In: Journal of Parasitology, Vol. 91, No. 6, 01.12.2005, p. 1509-1511.

Research output: Contribution to journalArticle

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