Pea cytochrome oxidase subunit II gene has no intron and generates two mRNA transcripts with different 5′-termini

Eunpyo Moon, Teh-hui Kao, Ray Wu

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We have isolated and sequenced the cytochrome oxidase subunit II (COII) gene from pea mitochondria. The coding sequence (777 bp) shows over 90% homology to the COII genes from three monocotyledonous plants (rice, maize and wheat) and one dicotyledonous plant (Oenothera berteriana) Several codons are deleted, however, in the pea COII gene. Of interest is the deletion in pea of the last three codons, including the stop codon, found at the 3′ end of the other four COII genes. Instead, a new stop codon has been created due to a single-base substitution at the 13th bp downstream from the position of the original stop codon. This pea gene does not contain an intron which is found in all three monocots. Two distinct 5′ termini of the pea COII transcripts have been identified by S1 nuclease mapping, one at 285 bp (site I) and the other at 302 bp (site II) upstream from the ATG codon. They are located at two identical sites within nearly perfect direct repeats. Transcripts with the 5′ end corresponding to site I occur five time more frequently than those with the 5′ end corresponding to site II. Both transcripts have the same 3′ terminus which has been mapped to be at 193 to 195 bp downstream from the stop codon.

Original languageEnglish (US)
Pages (from-to)3195-3212
Number of pages18
JournalNucleic acids research
Volume13
Issue number9
DOIs
StatePublished - May 10 1985

Fingerprint

Peas
Electron Transport Complex IV
Introns
Terminator Codon
Messenger RNA
Codon
Genes
Oenothera
Nucleic Acid Repetitive Sequences
Triticum
Zea mays
Mitochondria

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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title = "Pea cytochrome oxidase subunit II gene has no intron and generates two mRNA transcripts with different 5′-termini",
abstract = "We have isolated and sequenced the cytochrome oxidase subunit II (COII) gene from pea mitochondria. The coding sequence (777 bp) shows over 90{\%} homology to the COII genes from three monocotyledonous plants (rice, maize and wheat) and one dicotyledonous plant (Oenothera berteriana) Several codons are deleted, however, in the pea COII gene. Of interest is the deletion in pea of the last three codons, including the stop codon, found at the 3′ end of the other four COII genes. Instead, a new stop codon has been created due to a single-base substitution at the 13th bp downstream from the position of the original stop codon. This pea gene does not contain an intron which is found in all three monocots. Two distinct 5′ termini of the pea COII transcripts have been identified by S1 nuclease mapping, one at 285 bp (site I) and the other at 302 bp (site II) upstream from the ATG codon. They are located at two identical sites within nearly perfect direct repeats. Transcripts with the 5′ end corresponding to site I occur five time more frequently than those with the 5′ end corresponding to site II. Both transcripts have the same 3′ terminus which has been mapped to be at 193 to 195 bp downstream from the stop codon.",
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Pea cytochrome oxidase subunit II gene has no intron and generates two mRNA transcripts with different 5′-termini. / Moon, Eunpyo; Kao, Teh-hui; Wu, Ray.

In: Nucleic acids research, Vol. 13, No. 9, 10.05.1985, p. 3195-3212.

Research output: Contribution to journalArticle

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AB - We have isolated and sequenced the cytochrome oxidase subunit II (COII) gene from pea mitochondria. The coding sequence (777 bp) shows over 90% homology to the COII genes from three monocotyledonous plants (rice, maize and wheat) and one dicotyledonous plant (Oenothera berteriana) Several codons are deleted, however, in the pea COII gene. Of interest is the deletion in pea of the last three codons, including the stop codon, found at the 3′ end of the other four COII genes. Instead, a new stop codon has been created due to a single-base substitution at the 13th bp downstream from the position of the original stop codon. This pea gene does not contain an intron which is found in all three monocots. Two distinct 5′ termini of the pea COII transcripts have been identified by S1 nuclease mapping, one at 285 bp (site I) and the other at 302 bp (site II) upstream from the ATG codon. They are located at two identical sites within nearly perfect direct repeats. Transcripts with the 5′ end corresponding to site I occur five time more frequently than those with the 5′ end corresponding to site II. Both transcripts have the same 3′ terminus which has been mapped to be at 193 to 195 bp downstream from the stop codon.

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