Pedigree assessment using RAPD-DGGE in cereal crop species

Ismail Dweikat, Sally Mackenzie, Morris Levy, Herbert Ohm

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The introduction of molecular biology methodologies to plant improvement programs offers an invaluable opportunity for extensive germplasm characterization. However, the detection of adequate DNA polymorphism in self-pollinating species remains on obstacle. We have optimized a denaturing-gradient-gel electrophoresis (DGGE) system which, when used in combination with random amplified polymorphic DNA (RAPD) analysis, greatly facilitates the detection of reproducible DNA polymorphism among closely related plant lines. We have used this approach to estimate pedigree relationships among a spectrum of plant materials in wheat, barley and oat. Based on analysis with one or two primers, we were able to distinguish soft from hard winter wheat, and 2-rowed from 6-rowed barley. Further analysis with additional primers allowed resolution of polymorpisms even among closely related lines in highly selected populations. We placed 17 cultivars of oat into two distinct clusters that differed significantly from previous oat pedigree assessments. We believe that DGGE-RAPD is a superior method for detecting DNA polymorphism when compared to RFLP, agarose-RAPD, or polyacrylamide-RAPD methods.

Original languageEnglish (US)
Pages (from-to)497-505
Number of pages9
JournalTheoretical And Applied Genetics
Volume85
Issue number5
DOIs
StatePublished - Jan 1 1993

Fingerprint

random amplified polymorphic DNA technique
Denaturing Gradient Gel Electrophoresis
denaturing gradient gel electrophoresis
Pedigree
grain crops
pedigree
oats
DNA
genetic polymorphism
barley
Hordeum
polyacrylamide
Triticum
pollinators
molecular biology
agarose
winter wheat
restriction fragment length polymorphism
germplasm
methodology

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Dweikat, Ismail ; Mackenzie, Sally ; Levy, Morris ; Ohm, Herbert. / Pedigree assessment using RAPD-DGGE in cereal crop species. In: Theoretical And Applied Genetics. 1993 ; Vol. 85, No. 5. pp. 497-505.
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Pedigree assessment using RAPD-DGGE in cereal crop species. / Dweikat, Ismail; Mackenzie, Sally; Levy, Morris; Ohm, Herbert.

In: Theoretical And Applied Genetics, Vol. 85, No. 5, 01.01.1993, p. 497-505.

Research output: Contribution to journalArticle

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